Vav synergizes with protein kinase CΘ to mediate IL-4 gene expression in response to CD28 costimulation in T cells
The secretion of IL-4, which displays many important immunoregulatory functions, is restricted to cells of the Th2 subtype. In this study, we investigated the early signaling events leading to the activation of IL-4 transcription. Vav, the protein kinase C (PKC) isoform Θ, and the adaptor protein SL...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
April 1, 2000
|
| In: |
The journal of immunology
Year: 2000, Jahrgang: 164, Heft: 7, Pages: 3829-3836 |
| ISSN: | 1550-6606 |
| DOI: | 10.4049/jimmunol.164.7.3829 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.4049/jimmunol.164.7.3829 Verlag, lizenzpflichtig, Volltext: https://www.jimmunol.org/content/164/7/3829 
|
| Verfasserangaben: | Steffen P. Hehner, Min Li-Weber, Marco Giaisi, Wulf Dröge, Peter H. Krammer, and M. Lienhard Schmitz |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 174721034X | ||
| 003 | DE-627 | ||
| 005 | 20220819093949.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210203s2000 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.4049/jimmunol.164.7.3829 |2 doi | |
| 035 | |a (DE-627)174721034X | ||
| 035 | |a (DE-599)KXP174721034X | ||
| 035 | |a (OCoLC)1341390280 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Hehner, Steffen Peter |d 1971- |e VerfasserIn |0 (DE-588)122028260 |0 (DE-627)081691718 |0 (DE-576)182356566 |4 aut | |
| 245 | 1 | 0 | |a Vav synergizes with protein kinase CΘ to mediate IL-4 gene expression in response to CD28 costimulation in T cells |c Steffen P. Hehner, Min Li-Weber, Marco Giaisi, Wulf Dröge, Peter H. Krammer, and M. Lienhard Schmitz |
| 246 | 3 | 3 | |a Vav synergizes with protein kinase C theta to mediate IL-4 gene expression in response to CD28 costimulation in T cells |
| 264 | 1 | |c April 1, 2000 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Im Titel ist theta als griechischer Buchstabe dargestellt | ||
| 500 | |a Gesehen am 03.02.2021 | ||
| 520 | |a The secretion of IL-4, which displays many important immunoregulatory functions, is restricted to cells of the Th2 subtype. In this study, we investigated the early signaling events leading to the activation of IL-4 transcription. Vav, the protein kinase C (PKC) isoform Θ, and the adaptor protein SLP76 (SH2-domain-containing leukocyte protein of 76 kDa), induced transcription from the IL-4 promoter. Vav and PKCΘ synergistically activated human IL-4 promoter transcription and IL-4 mRNA production and were found to be constitutively associated in vivo. CD3/CD28-induced IL-4 transcription was inhibited upon coexpression of dominant negative forms of Vav, the adaptor proteins LAT (linker for activation of T cells) and SLP76, PKCΘ, and components of the pathways leading to the activation of c-Jun N-terminal kinase (mitogen-activated protein kinase kinase 7 (MKK7), mixed lineage kinase 3 (MLK3)) and NF-κB (IκB kinase α and IκB kinase β). The Vav/PKCΘ-mediated synergistic activation of IL-4 transcription was not inhibited by cyclosporin A. Three independent experimental approaches revealed that Vav/PKCΘ-derived signals selectively target the P1 and positive regulatory element (PRE)-I elements contained within the human IL-4 promoter. Vav/PKCΘ strongly activated a luciferase reporter construct controlled by trimerized P1 or PRE-I elements and furthermore stimulated DNA binding of nuclear proteins to the P1 and PRE-I elements. Vav/PKCΘ-induced transcription from the IL-4 promoter was almost completely abrogated by mutation of either the P1 or the PRE-I element within the entire IL-4 promoter. | ||
| 700 | 1 | |a Li-Weber, Min |e VerfasserIn |0 (DE-588)1157205399 |0 (DE-627)1021010324 |0 (DE-576)502732695 |4 aut | |
| 700 | 1 | |a Dröge, Wulf |d -2011 |e VerfasserIn |0 (DE-588)1178587789 |0 (DE-627)1049465539 |0 (DE-576)517837528 |4 aut | |
| 700 | 1 | |a Krammer, Peter H. |d 1946- |e VerfasserIn |0 (DE-588)121766373 |0 (DE-627)081520255 |0 (DE-576)292873417 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The journal of immunology |d Rockville, Md. : American Association of Immunologists, 1916 |g 164(2000), 7, Seite 3829-3836 |h Online-Ressource |w (DE-627)269535217 |w (DE-600)1475085-5 |w (DE-576)079963838 |x 1550-6606 |7 nnas |a Vav synergizes with protein kinase CΘ to mediate IL-4 gene expression in response to CD28 costimulation in T cells |
| 773 | 1 | 8 | |g volume:164 |g year:2000 |g number:7 |g pages:3829-3836 |g extent:8 |a Vav synergizes with protein kinase CΘ to mediate IL-4 gene expression in response to CD28 costimulation in T cells |
| 856 | 4 | 0 | |u https://doi.org/10.4049/jimmunol.164.7.3829 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.jimmunol.org/content/164/7/3829 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210203 | ||
| 993 | |a Article | ||
| 994 | |a 2000 | ||
| 998 | |g 121766373 |a Krammer, Peter H. |m 121766373:Krammer, Peter H. |d 50000 |e 50000PK121766373 |k 0/50000/ |p 5 | ||
| 998 | |g 1178587789 |a Dröge, Wulf |m 1178587789:Dröge, Wulf |d 50000 |e 50000PD1178587789 |k 0/50000/ |p 4 | ||
| 998 | |g 1157205399 |a Li-Weber, Min |m 1157205399:Li-Weber, Min |d 50000 |e 50000PL1157205399 |k 0/50000/ |p 2 | ||
| 999 | |a KXP-PPN174721034X |e 384833609X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"titleAlt":[{"title":"Vav synergizes with protein kinase C theta to mediate IL-4 gene expression in response to CD28 costimulation in T cells"}],"recId":"174721034X","language":["eng"],"note":["Im Titel ist theta als griechischer Buchstabe dargestellt","Gesehen am 03.02.2021"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title_sort":"Vav synergizes with protein kinase CΘ to mediate IL-4 gene expression in response to CD28 costimulation in T cells","title":"Vav synergizes with protein kinase CΘ to mediate IL-4 gene expression in response to CD28 costimulation in T cells"}],"person":[{"role":"aut","display":"Hehner, Steffen Peter","roleDisplay":"VerfasserIn","given":"Steffen Peter","family":"Hehner"},{"family":"Li-Weber","given":"Min","display":"Li-Weber, Min","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Dröge, Wulf","roleDisplay":"VerfasserIn","role":"aut","family":"Dröge","given":"Wulf"},{"role":"aut","display":"Krammer, Peter H.","roleDisplay":"VerfasserIn","given":"Peter H.","family":"Krammer"}],"relHost":[{"title":[{"title_sort":"journal of immunology","title":"The journal of immunology"}],"language":["eng"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"American Association of Immunologists","role":"isb"}],"recId":"269535217","disp":"Vav synergizes with protein kinase CΘ to mediate IL-4 gene expression in response to CD28 costimulation in T cellsThe journal of immunology","note":["Gesehen am 18.08.2025"],"type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"text":"164(2000), 7, Seite 3829-3836","volume":"164","extent":"8","year":"2000","pages":"3829-3836","issue":"7"},"pubHistory":["1.1916 -"],"name":{"displayForm":["American Association of Immunologists"]},"id":{"issn":["1550-6606"],"eki":["269535217"],"zdb":["1475085-5"]},"origin":[{"dateIssuedKey":"2025","publisher":"Oxford University Press ; Soc. ; American Association of Immunologists","dateIssuedDisp":"2025-","publisherPlace":"Oxford ; Bethesda, Md. ; Rockville, Md."}],"physDesc":[{"extent":"Online-Ressource"}]}],"physDesc":[{"extent":"8 S."}],"id":{"eki":["174721034X"],"doi":["10.4049/jimmunol.164.7.3829"]},"origin":[{"dateIssuedKey":"2000","dateIssuedDisp":"April 1, 2000"}],"name":{"displayForm":["Steffen P. Hehner, Min Li-Weber, Marco Giaisi, Wulf Dröge, Peter H. Krammer, and M. Lienhard Schmitz"]}} | ||
| SRT | |a HEHNERSTEFVAVSYNERGI1200 | ||