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Selective modulation of cell response on engineered fractal silicon substrates

A plethora of work has been dedicated to the analysis of cell behavior on substrates with ordered topographical features. However, the natural cell microenvironment is characterized by biomechanical cues organized over multiple scales. Here, randomly rough, self-affinefractal surfaces are generated...

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Bibliographic Details
Main Authors: Gentile, Francesco (Author) , Medda, Rebecca (Author) , Cheng, Ling (Author) , Battista, Edmondo (Author) , Scopelliti, Pasquale E. (Author) , Milani, Paolo (Author) , Cavalcanti-Adam, Elisabetta A. (Author) , Decuzzi, Paolo (Author)
Format: Article (Journal)
Language:English
Published: 15 March 2013
In: Scientific reports
Year: 2013, Volume: 3
ISSN:2045-2322
DOI:10.1038/srep01461
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/srep01461
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/srep01461
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Author Notes:Francesco Gentile, Rebecca Medda, Ling Cheng, Edmondo Battista, Pasquale E. Scopelliti, Paolo Milani, Elisabetta A. Cavalcanti-Adam & Paolo Decuzzi
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Summary:A plethora of work has been dedicated to the analysis of cell behavior on substrates with ordered topographical features. However, the natural cell microenvironment is characterized by biomechanical cues organized over multiple scales. Here, randomly rough, self-affinefractal surfaces are generated out of silicon,where roughness Ra and fractal dimension Df are independently controlled. The proliferation rates, the formation of adhesion structures and the morphology of 3T3 murine fibroblasts are monitored over six different substrates. The proliferation rate is maximized on surfaces with moderate roughness (Ra ~ 40 nm) and large fractal dimension (Df ~ 2.4); whereas adhesion structures are wider and more stable on substrates with higher roughness (Ra ~ 50 nm) and lower fractal dimension (Df ~ 2.2). Higher proliferation occurson substrates exhibiting densely packed and sharp peaks, whereas more regular ridges favor adhesion. These results suggest that randomly roughtopographies can selectively modulate cell behavior.
Item Description:Gesehen am 04.02.2020
Physical Description:Online Resource
ISSN:2045-2322
DOI:10.1038/srep01461

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