Pharmacokinetics and metabolism of the methyl-branched fatty acid (BMIPP) in animals and humans
<p>The aim of this study was to further characterize the major metabolite of 15-(p-iodophenyl)-3-(R,S)-methylpentadecanoic acid (BMIPP). <b>Methods:</b> Radioactive components of <sup>131</sup>I-BMIPP were evaluated in Langendorff-perfused rat hearts, as well as in bloo...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
1999
|
| In: |
Journal of nuclear medicine
Year: 1999, Jahrgang: 40, Heft: 9, Pages: 1484-1491 |
| ISSN: | 2159-662X |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://jnm.snmjournals.org/content/40/9/1484
|
| Verfasserangaben: | Joachim Kropp, Michael Eisenhut, Kathleen R. Ambrose, Furn F. (Russ) Knapp and Wolf-Gunter Franke |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 174789916X | ||
| 003 | DE-627 | ||
| 005 | 20230426111248.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210209s1999 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)174789916X | ||
| 035 | |a (DE-599)KXP174789916X | ||
| 035 | |a (OCoLC)1341392156 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Kropp, Joachim |d 1950- |e VerfasserIn |0 (DE-588)1127749439 |0 (DE-627)882203606 |0 (DE-576)485363879 |4 aut | |
| 245 | 1 | 0 | |a Pharmacokinetics and metabolism of the methyl-branched fatty acid (BMIPP) in animals and humans |c Joachim Kropp, Michael Eisenhut, Kathleen R. Ambrose, Furn F. (Russ) Knapp and Wolf-Gunter Franke |
| 264 | 1 | |c 1999 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 09.02.2021 | ||
| 520 | |a <p>The aim of this study was to further characterize the major metabolite of 15-(p-iodophenyl)-3-(R,S)-methylpentadecanoic acid (BMIPP). <b>Methods:</b> Radioactive components of <sup>131</sup>I-BMIPP were evaluated in Langendorff-perfused rat hearts, as well as in blood samples from 20 patients after injection of <sup>123</sup>I-BMIPP. Rat hearts were perfused with pH 7.4 Krebs-Henseleit buffer with or without 0.4 mmoI/L bovine serum albumin (BSA) or 0.4 mmoI/L palmitate. Lipids were Folch extracted and hydrolyzed from samples of the outflow, as well as from homogenized hearts. Radioactive components were determined by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analyses. The major metabolite of BMIPP was then further characterized by electrospray mass spectrometry. <b>Results:</b> The rat heart perfusate showed one major polar metabolite observed by TLC (R<sub>f</sub> = 0.35; solvent = benzene-dioxane-acetic acid 80:18:2). The addition of BSA/palmitate to the perfusate buffer significantly increased back diffusion of BMIPP (R<sup>f</sup> = 0.55), as well as reduced BMIPP uptake and metabolism. The major metabolite was identified by mass spectral analysis as 2-(piodophenyl) acetic acid (IPC<sub>2</sub>). From TLC and HPLC analyses of the serum lipids obtained from patients, the same metabolite could be identified with levels increasing overtime (0%, 5.2% and 11.8% of the injected dose; 3 min, 20 min and 3 h postinjection, respectively). In addition to the identification of unmetabolized BMIPP (53.9%), the rat heart lipid hydrolysate also contained α-methyl-14-(p-iodophenyl)tet radecanoic acid (20.8%), 12-(p-iodophenyl)-substituted-dodecanoic (17.1%), -hexanoic acid (5.2%) and IPC<sub>2</sub> (1.1%). <b>Conclusion:</b> The animal results show the complexity of uptake, metabolism and release of BMIPP from which a part is metabolized through α- and subsequent ß-oxidation to the final IPC<sub>2</sub> metabolite as confirmed by mass spectral analysis. The results from patient studies suggest that the slow myocardial washout observed in vivo after intravenous administration of BMIPP may represent a similar process, because both unmetabolized BMIPP and the final metabolite were also identified in serum samples.</p> | ||
| 700 | 1 | |a Eisenhut, Michael |e VerfasserIn |0 (DE-588)1113306122 |0 (DE-627)867174501 |0 (DE-576)160846080 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of nuclear medicine |d New York, NY : Soc., 1964 |g 40(1999), 9, Seite 1484-1491 |h Online-Ressource |w (DE-627)325793603 |w (DE-600)2040222-3 |w (DE-576)09475277X |x 2159-662X |7 nnas |a Pharmacokinetics and metabolism of the methyl-branched fatty acid (BMIPP) in animals and humans |
| 773 | 1 | 8 | |g volume:40 |g year:1999 |g number:9 |g pages:1484-1491 |g extent:8 |a Pharmacokinetics and metabolism of the methyl-branched fatty acid (BMIPP) in animals and humans |
| 856 | 4 | 0 | |u https://jnm.snmjournals.org/content/40/9/1484 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210209 | ||
| 993 | |a Article | ||
| 994 | |a 1999 | ||
| 998 | |g 1113306122 |a Eisenhut, Michael |m 1113306122:Eisenhut, Michael |d 50000 |e 50000PE1113306122 |k 0/50000/ |p 2 | ||
| 999 | |a KXP-PPN174789916X |e 3850439704 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"8 S."}],"relHost":[{"title":[{"subtitle":"JNM","title":"Journal of nuclear medicine","title_sort":"Journal of nuclear medicine"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Pharmacokinetics and metabolism of the methyl-branched fatty acid (BMIPP) in animals and humansJournal of nuclear medicine","note":["Gesehen am 13.12.2021"],"corporate":[{"role":"isb","display":"Society of Nuclear Medicine","roleDisplay":"Herausgebendes Organ"}],"language":["eng"],"recId":"325793603","pubHistory":["Nachgewiesen 5.1964 -"],"titleAlt":[{"title":"JNM"}],"part":{"year":"1999","pages":"1484-1491","issue":"9","text":"40(1999), 9, Seite 1484-1491","volume":"40","extent":"8"},"name":{"displayForm":["Society of Nuclear Medicine"]},"origin":[{"publisherPlace":"New York, NY","publisher":"Soc.","dateIssuedKey":"1964","dateIssuedDisp":"1964-"}],"id":{"eki":["325793603"],"zdb":["2040222-3"],"issn":["2159-662X","1535-5667"]},"physDesc":[{"extent":"Online-Ressource"}]}],"name":{"displayForm":["Joachim Kropp, Michael Eisenhut, Kathleen R. Ambrose, Furn F. (Russ) Knapp and Wolf-Gunter Franke"]},"origin":[{"dateIssuedDisp":"1999","dateIssuedKey":"1999"}],"id":{"eki":["174789916X"]},"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 09.02.2021"],"language":["eng"],"recId":"174789916X","person":[{"role":"aut","roleDisplay":"VerfasserIn","display":"Kropp, Joachim","given":"Joachim","family":"Kropp"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Eisenhut, Michael","given":"Michael","family":"Eisenhut"}],"title":[{"title_sort":"Pharmacokinetics and metabolism of the methyl-branched fatty acid (BMIPP) in animals and humans","title":"Pharmacokinetics and metabolism of the methyl-branched fatty acid (BMIPP) in animals and humans"}]} | ||
| SRT | |a KROPPJOACHPHARMACOKI1999 | ||