Elevated expression of genes assigned to NF-κB and apoptotic pathways in human periodontal ligament fibroblasts following mechanical stretch
There is growing evidence that apoptosis involves the nuclear transcription factor NF-κB in conjunction with related genes. However, in the context of mechanical orthodontic forces, force-sensing target genes assigned to pathways of NF-κB and apoptosis have not been fully characterised. To contribut...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
6 March 2007
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| In: |
Cell & tissue research
Year: 2007, Volume: 328, Issue: 3, Pages: 537-548 |
| ISSN: | 1432-0878 |
| DOI: | 10.1007/s00441-007-0382-x |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1007/s00441-007-0382-x Verlag, lizenzpflichtig, Volltext: https://link.springer.com/article/10.1007/s00441-007-0382-x 
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| Author Notes: | Nina Ritter, Eva Mussig, Thorsten Steinberg, Annette Kohl, Gerda Komposch, Pascal Tomakidi |
MARC
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| 520 | |a There is growing evidence that apoptosis involves the nuclear transcription factor NF-κB in conjunction with related genes. However, in the context of mechanical orthodontic forces, force-sensing target genes assigned to pathways of NF-κB and apoptosis have not been fully characterised. To contribute to the identification of putative target genes, we used cDNA arrays specific for NF-κB and apoptotic pathways and analysed elevated gene expression in primary human periodontal ligament fibroblasts (PDL-F) after a 6 h application of mechanical force. Among several identified genes (including several caspases), interleukin-1β (IL-1β) and NF-κB displayed significantly higher expression on the NF-κB array, whereas higher expression was obtained for BCL2-antagonist of cell death (BAD), member 6 of the TNF-receptor superfamily (FAS) and CASP2 and RIPK1 domain-containing adaptor with death domain (CRADD) on the apoptosis array. Based on a defined cut-off level of a more than 1.5-fold higher expression, this significance in elevated gene expression was corroborated by reverse transcription/polymerase chain reaction (RT-PCR). Here, semi-quantitative (sq) PCR revealed a more pronounced elevation of mRNA gene expression in PDL-F after 6 h of stretch, when compared with 12 h. Moreover, the elevation after 6 h as observed by sq-PCR was convergent with quantitative PCR (q-PCR). q-PCR yielded levels of 5.8-fold higher relative gene expression for IL-1β and 1.7-fold for NF-κB, whereas that computed for BAD indicated a 5.2-fold, for CRADD a 2.1-fold and for FAS a 2.0-fold higher expression. The data obtained from the expression analysis thus indicate a stretch-induced transcriptional elevation of genes assigned to the NF-κB and apoptotic pathways. This elevation may render them target candidates for being addressed by mechanical orthodontic forces. | ||
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