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Siglec-1-positive plasmacytoid dendritic cells (pDCs) in human peripheral blood: a semi-mature and myeloid-like subset imbalanced during protective and autoimmune responses

Plasmacytoid dendritic cells (pDCs) play a central role in the pathogenesis of systemic lupus erythematosus (SLE) as IFN-α producers and promoters of T-cell activation or tolerance. Here, we demonstrated by flow-cytometry and confocal microscopy that Siglec-1, a molecule involved in the regulation o...

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Main Authors: Wilhelm, Theresa Rita (Author) , Taddeo, Adriano (Author) , Winter, Oliver (Author) , Schulz, Axel Ronald (Author) , Mälzer, Julia-Nora (Author) , Domingo, Cristina (Author) , Biesen, Robert (Author) , Alexander, Tobias (Author) , Thiel, Andreas (Author) , Radbruch, Andreas (Author) , Hiepe, Falk (Author) , Gerl, Velia (Author)
Format: Article (Journal)
Language:English
Published: 2016
In: Clinical immunology
Year: 2015, Volume: 163, Pages: 42-51
ISSN:1521-7035
DOI:10.1016/j.clim.2015.12.001
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.clim.2015.12.001
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S152166161530070X
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Author Notes:Theresa R. Wilhelm, Adriano Taddeo, Oliver Winter, Axel Ronald Schulz, Julia-Nora Mälzer, Cristina Domingo, Robert Biesen, Tobias Alexander, Andreas Thiel, Andreas Radbruch, Falk Hiepe, Velia Gerl
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Summary:Plasmacytoid dendritic cells (pDCs) play a central role in the pathogenesis of systemic lupus erythematosus (SLE) as IFN-α producers and promoters of T-cell activation or tolerance. Here, we demonstrated by flow-cytometry and confocal microscopy that Siglec-1, a molecule involved in the regulation of adaptive immunoresponses, is expressed in a subset of semi-mature, myeloid-like pDCs in human blood. These pDCs express lower BDCA-2 and CD123 and higher HLA-DR and CD11c than Siglec-1-negative pDCs and do not produce IFN-α via TLR7/TLR9 engagement. In vitro, Siglec-1 expression was induced in Siglec-1-negative pDCs by influenza virus. Proportions of Siglec-1-positive/Siglec-1-negative pDCs were higher in SLE than in healthy controls and correlated with disease activity. Healthy donors immunized with yellow fever vaccine YFV-17D displayed different kinetics of the two pDC subsets during protective immune response. PDCs can be subdivided into two subsets according to Siglec-1 expression. These subsets may play specific roles in (auto)immune responses.
Item Description:Available online 7 December 2015
Gesehen am 11.02.2021
Physical Description:Online Resource
ISSN:1521-7035
DOI:10.1016/j.clim.2015.12.001

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