Immune evasion of Borrelia burgdorferi by acquisition of human complement regulators FHL-1/reconectin and Factor H
To understand immune evasion mechanisms of Borrelia burgdorferi we compared serum-resistant B. afzelii and serum-sensitive B. garinii isolates for their capacity toacquire human complement regulators. Here we demonstrate that the two borrelial genospecies show different binding of the two important...
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| Hauptverfasser: | , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
16 May 2001
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| In: |
European journal of immunology
Year: 2001, Jahrgang: 31, Heft: 6, Pages: 1674-1684 |
| ISSN: | 1521-4141 |
| DOI: | 10.1002/1521-4141(200106)31:6<1674::aid-immu1674>3.0.co;2-2 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/1521-4141(200106)31:6<1674::aid-immu1674>3.0.co;2-2 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1521-4141(200106)31:6<1674::aid-immu1674>3.0.co;2-2 
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| Verfasserangaben: | Peter Kraiczy, Christine Skerka, Michael Kirschfink, Volker Brade and Peter F. Zipfel |
MARC
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| 245 | 1 | 0 | |a Immune evasion of Borrelia burgdorferi by acquisition of human complement regulators FHL-1/reconectin and Factor H |c Peter Kraiczy, Christine Skerka, Michael Kirschfink, Volker Brade and Peter F. Zipfel |
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| 520 | |a To understand immune evasion mechanisms of Borrelia burgdorferi we compared serum-resistant B. afzelii and serum-sensitive B. garinii isolates for their capacity toacquire human complement regulators. Here we demonstrate that the two borrelial genospecies show different binding of the two important human complement regulators, FHL-1/reconectin and Factor H. All serum-resistant B. afzelii isolates bound FHL-1/reconectin and also Factor H, and all analyzed serum-sensitive B. garinii isolates showed no or a significantly lower binding activity. Using recombinant deletion mutants, the binding domains were localized to the C terminus of FHL-1/reconectin to short consensus repeats 5-7. The borrelial binding proteins were located in the surface of the bacteria as demonstrated by immunofluorescence staining of intact, serum-exposed bacteria and by enrichment of outer membrane proteins. The surface-attached complement regulators maintained complement regulatory activity as demonstrated in a cofactor assay. By ligand blotting two different borrelial binding proteins were identified that were responsible for the surface attachment of FHL-1/reconectin and Factor H. These borrelial complement regulators acquiring surface proteins (CRASP) were further characterized as either CRASP-1, a 27.5-kDa molecule which preferentially binds FHL-1/reconectin and which was present in all serum-resistant borreliae, or CRASP-2, a 20/21-kDa protein which interacts preferentially with Factor H and the expression of which was more restricted, being detected in four of the six isolates analyzed. In summary, we describe a new immune evasion mechanism of B. burgdorferi, as these bacteria acquire human complement regulators to control complement activation on their surface and to prevent formation of toxic activation products. | ||
| 650 | 4 | |a Adsorption | |
| 650 | 4 | |a Bacterial Proteins | |
| 650 | 4 | |a Binding Sites | |
| 650 | 4 | |a Blood Proteins | |
| 650 | 4 | |a Borrelia burgdorferi Group | |
| 650 | 4 | |a Complement Activation | |
| 650 | 4 | |a Complement C3b | |
| 650 | 4 | |a Complement C3b Inactivator Proteins | |
| 650 | 4 | |a Complement Factor H | |
| 650 | 4 | |a Humans | |
| 650 | 4 | |a Lyme Disease | |
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