GTP regulates the microtubule nucleation activity of γ-tubulin
Both subunits of αβ-tubulin that comprise the core components of microtubules bind GTP. GTP binding to α-tubulin has a structural role, whereas β-tubulin binds and hydrolyses GTP to regulate microtubule dynamics. γ-tubulin, another member of the tubulin superfamily that seeds microtubule nucleation...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
27 October 2013
|
| In: |
Nature cell biology
Year: 2013, Jahrgang: 15, Heft: 11, Pages: 1317-1327 |
| ISSN: | 1476-4679 |
| DOI: | 10.1038/ncb2863 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/ncb2863 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/ncb2863 
|
| Verfasserangaben: | Linda Gombos, Annett Neuner, Mykhaylo Berynskyy, Luca L. Fava, Rebecca C. Wade, Carsten Sachse and Elmar Schiebel |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1748531921 | ||
| 003 | DE-627 | ||
| 005 | 20220819111503.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210217s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/ncb2863 |2 doi | |
| 035 | |a (DE-627)1748531921 | ||
| 035 | |a (DE-599)KXP1748531921 | ||
| 035 | |a (OCoLC)1341393744 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Gombos, Linda |e VerfasserIn |0 (DE-588)1167894200 |0 (DE-627)1031413693 |0 (DE-576)511333617 |4 aut | |
| 245 | 1 | 0 | |a GTP regulates the microtubule nucleation activity of γ-tubulin |c Linda Gombos, Annett Neuner, Mykhaylo Berynskyy, Luca L. Fava, Rebecca C. Wade, Carsten Sachse and Elmar Schiebel |
| 246 | 3 | 3 | |a GTP regulates the microtubule nucleation activity of gamma-tubulin |
| 264 | 1 | |c 27 October 2013 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 17.02.2020 | ||
| 520 | |a Both subunits of αβ-tubulin that comprise the core components of microtubules bind GTP. GTP binding to α-tubulin has a structural role, whereas β-tubulin binds and hydrolyses GTP to regulate microtubule dynamics. γ-tubulin, another member of the tubulin superfamily that seeds microtubule nucleation at microtubule-organizing centres, also binds GTP; however, the importance of this association remains elusive. To address the role of GTP binding to γ-tubulin, we systematically mutagenized the GTP contact residues in the yeast γ-tubulin Tub4. Tub4GTP-mutant proteins that exhibited greatly reduced GTP affinity still assembled into the small γ-tubulin complex. However, tub4GTP mutants were no longer viable, and had defects in interaction between γ-tubulin and αβ-tubulin, decreased microtubule nucleation and defects in microtubule organization. In vitro and in vivo data show that only γ-tubulin loaded with GTP nucleates microtubules. Our results suggest that GTP recruitment to γ-tubulin enhances its interaction with αβ-tubulin similarly to GTP recruitment to β-tubulin. | ||
| 700 | 1 | |a Neuner, Annett |d 1970- |e VerfasserIn |0 (DE-588)130046256 |0 (DE-627)488789702 |0 (DE-576)297967452 |4 aut | |
| 700 | 1 | |a Berinski, Michael |d 1986- |e VerfasserIn |0 (DE-588)1079189106 |0 (DE-627)840607792 |0 (DE-576)452260612 |4 aut | |
| 700 | 1 | |a Fava, Luca L. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wade, Rebecca C. |e VerfasserIn |0 (DE-588)102801774X |0 (DE-627)730136000 |0 (DE-576)276591402 |4 aut | |
| 700 | 1 | |a Sachse, Carsten |d 1978- |e VerfasserIn |0 (DE-588)133507254 |0 (DE-627)69151898X |0 (DE-576)299894509 |4 aut | |
| 700 | 1 | |a Schiebel, Elmar |d 1960- |e VerfasserIn |0 (DE-588)1034622595 |0 (DE-627)746029462 |0 (DE-576)165868597 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature cell biology |d New York, NY : Nature America, 1999 |g 15(2013), 11, Seite 1317-1327 |h Online-Ressource |w (DE-627)303393726 |w (DE-600)1494945-3 |w (DE-576)08088718X |x 1476-4679 |7 nnas |a GTP regulates the microtubule nucleation activity of γ-tubulin |
| 773 | 1 | 8 | |g volume:15 |g year:2013 |g number:11 |g pages:1317-1327 |g extent:11 |a GTP regulates the microtubule nucleation activity of γ-tubulin |
| 856 | 4 | 0 | |u https://doi.org/10.1038/ncb2863 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/ncb2863 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210217 | ||
| 993 | |a Article | ||
| 994 | |a 2013 | ||
| 998 | |g 1034622595 |a Schiebel, Elmar |m 1034622595:Schiebel, Elmar |d 700000 |d 706000 |e 700000PS1034622595 |e 706000PS1034622595 |k 0/700000/ |k 1/700000/706000/ |p 7 |y j | ||
| 998 | |g 133507254 |a Sachse, Carsten |m 133507254:Sachse, Carsten |p 6 | ||
| 998 | |g 102801774X |a Wade, Rebecca C. |m 102801774X:Wade, Rebecca C. |d 140000 |e 140000PW102801774X |k 0/140000/ |p 5 | ||
| 998 | |g 1079189106 |a Berinski, Michael |m 1079189106:Berinski, Michael |p 3 | ||
| 998 | |g 130046256 |a Neuner, Annett |m 130046256:Neuner, Annett |d 700000 |d 706000 |e 700000PN130046256 |e 706000PN130046256 |k 0/700000/ |k 1/700000/706000/ |p 2 | ||
| 998 | |g 1167894200 |a Gombos, Linda |m 1167894200:Gombos, Linda |p 1 |x j | ||
| 999 | |a KXP-PPN1748531921 |e 3857008113 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title_sort":"GTP regulates the microtubule nucleation activity of γ-tubulin","title":"GTP regulates the microtubule nucleation activity of γ-tubulin"}],"recId":"1748531921","type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"name":{"displayForm":["Linda Gombos, Annett Neuner, Mykhaylo Berynskyy, Luca L. Fava, Rebecca C. Wade, Carsten Sachse and Elmar Schiebel"]},"note":["Gesehen am 17.02.2020"],"person":[{"roleDisplay":"VerfasserIn","family":"Gombos","role":"aut","display":"Gombos, Linda","given":"Linda"},{"given":"Annett","display":"Neuner, Annett","role":"aut","roleDisplay":"VerfasserIn","family":"Neuner"},{"display":"Berinski, Michael","role":"aut","given":"Michael","roleDisplay":"VerfasserIn","family":"Berinski"},{"given":"Luca L.","display":"Fava, Luca L.","role":"aut","roleDisplay":"VerfasserIn","family":"Fava"},{"role":"aut","display":"Wade, Rebecca C.","given":"Rebecca C.","family":"Wade","roleDisplay":"VerfasserIn"},{"role":"aut","display":"Sachse, Carsten","given":"Carsten","family":"Sachse","roleDisplay":"VerfasserIn"},{"given":"Elmar","role":"aut","display":"Schiebel, Elmar","roleDisplay":"VerfasserIn","family":"Schiebel"}],"physDesc":[{"extent":"11 S."}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 02.03.05"],"origin":[{"dateIssuedDisp":"1999-","publisher":"Nature America","dateIssuedKey":"1999","publisherPlace":"New York, NY"}],"titleAlt":[{"title":"Cell biology"}],"id":{"zdb":["1494945-3"],"issn":["1476-4679"],"eki":["303393726"]},"recId":"303393726","title":[{"title_sort":"Nature cell biology","title":"Nature cell biology"}],"disp":"GTP regulates the microtubule nucleation activity of γ-tubulinNature cell biology","part":{"extent":"11","volume":"15","issue":"11","year":"2013","text":"15(2013), 11, Seite 1317-1327","pages":"1317-1327"},"language":["eng"],"type":{"media":"Online-Ressource","bibl":"periodical"},"pubHistory":["1.1999 -"]}],"id":{"eki":["1748531921"],"doi":["10.1038/ncb2863"]},"titleAlt":[{"title":"GTP regulates the microtubule nucleation activity of gamma-tubulin"}],"origin":[{"dateIssuedDisp":"27 October 2013","dateIssuedKey":"2013"}]} | ||
| SRT | |a GOMBOSLINDGTPREGULAT2720 | ||