Cover Image

Replicating parvoviruses that target colon cancer cells

The wnt signaling pathway is constitutively activated in colon tumors by mutations in the adenomatous polyposis coli and β-catenin genes. We have modified the minute virus of mice (MVM) P4 promoter to make it responsive to wnt signaling by inserting binding sites for the heterodimeric β-catenin/Tcf...

Full description

Saved in:
Bibliographic Details
Main Authors: Malerba, Maddalena (Author) , Daeffler, Laurent (Author) , Rommelaere, Jean (Author)
Format: Article (Journal)
Language:English
Published: June 15, 2003
In: Journal of virology
Year: 2003, Volume: 77, Issue: 12, Pages: 6683-6691
ISSN:1098-5514
DOI:10.1128/JVI.77.12.6683-6691.2003
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1128/JVI.77.12.6683-6691.2003
Verlag, lizenzpflichtig, Volltext: https://jvi.asm.org/content/77/12/6683
Get full text
Author Notes:M. Malerba, L. Daeffler, J. Rommelaere, and R.D. Iggo

MARC

LEADER 00000caa a2200000 c 4500
001 174861908X
003 DE-627
005 20220819112052.0
007 cr uuu---uuuuu
008 210218s2003 xx |||||o 00| ||eng c
024 7 |a 10.1128/JVI.77.12.6683-6691.2003  |2 doi 
035 |a (DE-627)174861908X 
035 |a (DE-599)KXP174861908X 
035 |a (OCoLC)1341393803 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Malerba, Maddalena  |e VerfasserIn  |0 (DE-588)1227637969  |0 (DE-627)1748619845  |4 aut 
245 1 0 |a Replicating parvoviruses that target colon cancer cells  |c M. Malerba, L. Daeffler, J. Rommelaere, and R.D. Iggo 
264 1 |c June 15, 2003 
300 |a 9 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 18.02.2021 
520 |a The wnt signaling pathway is constitutively activated in colon tumors by mutations in the adenomatous polyposis coli and β-catenin genes. We have modified the minute virus of mice (MVM) P4 promoter to make it responsive to wnt signaling by inserting binding sites for the heterodimeric β-catenin/Tcf transcription factor. In luciferase assays we can see up to 20-fold selectivity of Tcf mutant P4 promoters for cells with activated wnt signaling. Hybrid MVM/H-1 viruses containing Tcf mutant promoters were tested for NS1 expression, viral DNA replication, virus replication, and cytopathic effect on colon, lung, kidney, and cervical cancer cell lines. Activation of the wnt pathway by expression of ΔN-β-catenin increased NS1 expression and viral burst size in 293T and H1299 lung cancer cells, showing that the Tcf mutant P4 promoter can respond to wnt signals in the context of the virus. Compared to the parental virus, the burst size of the Tcf mutant viruses was reduced at least 1,000-fold in H1299, 293T, NB324K, and HeLa cells, which have inactive wnt signaling pathways. The burst size and cytopathic effect of the Tcf viruses was near wild-type levels in SW480 and Isreco1 colon cancer cell lines, which have high Tcf activity. The high specificity of these viruses should permit the development of H-1 virus-based vectors which combine high safety and greater efficacy in cancer therapy. 
700 1 |a Daeffler, Laurent  |e VerfasserIn  |0 (DE-588)1227507909  |0 (DE-627)174851234X  |4 aut 
700 1 |a Rommelaere, Jean  |d 1948-  |e VerfasserIn  |0 (DE-588)1201673909  |0 (DE-627)1685754767  |4 aut 
773 0 8 |i Enthalten in  |t Journal of virology  |d Baltimore, Md. : Soc., 1967  |g 77(2003), 12, Seite 6683-6691  |h Online-Ressource  |w (DE-627)303614609  |w (DE-600)1495529-5  |w (DE-576)08088766X  |x 1098-5514  |7 nnas  |a Replicating parvoviruses that target colon cancer cells 
773 1 8 |g volume:77  |g year:2003  |g number:12  |g pages:6683-6691  |g extent:9  |a Replicating parvoviruses that target colon cancer cells 
856 4 0 |u https://doi.org/10.1128/JVI.77.12.6683-6691.2003  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://jvi.asm.org/content/77/12/6683  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20210218 
993 |a Article 
994 |a 2003 
998 |g 1201673909  |a Rommelaere, Jean  |m 1201673909:Rommelaere, Jean  |d 50000  |e 50000PR1201673909  |k 0/50000/  |p 3 
998 |g 1227507909  |a Daeffler, Laurent  |m 1227507909:Daeffler, Laurent  |d 50000  |e 50000PD1227507909  |k 0/50000/  |p 2 
999 |a KXP-PPN174861908X  |e 385856382X 
BIB |a Y 
SER |a journal 
JSO |a {"physDesc":[{"extent":"9 S."}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"dateIssuedKey":"1967","publisher":"Soc.","dateIssuedDisp":"1967-","publisherPlace":"Baltimore, Md."}],"id":{"issn":["1098-5514"],"zdb":["1495529-5"],"eki":["303614609"]},"pubHistory":["1.1967 -"],"part":{"extent":"9","text":"77(2003), 12, Seite 6683-6691","volume":"77","pages":"6683-6691","issue":"12","year":"2003"},"note":["Gesehen am 13.12.2021"],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Replicating parvoviruses that target colon cancer cellsJournal of virology","corporate":[{"display":"American Society for Microbiology","roleDisplay":"Herausgebendes Organ","role":"isb"}],"language":["eng"],"recId":"303614609","title":[{"title":"Journal of virology","subtitle":"publ. by the American Society for Microbiology","title_sort":"Journal of virology"}]}],"name":{"displayForm":["M. Malerba, L. Daeffler, J. Rommelaere, and R.D. Iggo"]},"origin":[{"dateIssuedDisp":"June 15, 2003","dateIssuedKey":"2003"}],"id":{"eki":["174861908X"],"doi":["10.1128/JVI.77.12.6683-6691.2003"]},"note":["Gesehen am 18.02.2021"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"recId":"174861908X","person":[{"family":"Malerba","given":"Maddalena","display":"Malerba, Maddalena","roleDisplay":"VerfasserIn","role":"aut"},{"role":"aut","display":"Daeffler, Laurent","roleDisplay":"VerfasserIn","given":"Laurent","family":"Daeffler"},{"roleDisplay":"VerfasserIn","display":"Rommelaere, Jean","role":"aut","family":"Rommelaere","given":"Jean"}],"title":[{"title_sort":"Replicating parvoviruses that target colon cancer cells","title":"Replicating parvoviruses that target colon cancer cells"}]} 
SRT |a MALERBAMADREPLICATIN1520 

Similar Items