Organotypic co-cultures allow for immortalized human gingival keratinocytes to reconstitute a gingival epithelial phenotype in vitro
We report here that the organotypic co-culture (OCC) system allows for significant preservation of the tissue-specific phenotype of human gingival keratinocytes (IHGK) immortalized with the E6/E7 gene of the human papillomavirus type 16 (HPV16). The approach adopted is based on the OCC system facili...
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| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2006
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| In: |
Differentiation
Year: 2006, Volume: 74, Issue: 9, Pages: 622-637 |
| ISSN: | 1432-0436 |
| DOI: | 10.1111/j.1432-0436.2006.00099.x |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1111/j.1432-0436.2006.00099.x Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0301468109602486 |
| Author Notes: | Mariana Roesch-Ely, Thorsten Steinberg, F. Xavier Bosch, Eva Müssig, Noel Whitaker, Tina Wiest, Annette Kohl, Gerda Komposch, Pascal Tomakidi |
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| 520 | |a We report here that the organotypic co-culture (OCC) system allows for significant preservation of the tissue-specific phenotype of human gingival keratinocytes (IHGK) immortalized with the E6/E7 gene of the human papillomavirus type 16 (HPV16). The approach adopted is based on the OCC system facilitating spatially separated cell growth and cell-to-cell interactions via diffusible growth factors. | ||
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