The Hox transcription factor Ubx ensures somatic myogenesis by suppressing the mesodermal master regulator Twist

Early lineage-specific master regulators are essential for the specification of cell types. However, once cells are committed to a specific fate, it is critical to restrict the activity of such factors to enable differentiation. To date, it remains unclear how these factors are silenced. Using the D...

Full description

Saved in:
Bibliographic Details
Main Authors: Domsch, Katrin (Author) , Schröder, Julia (Author) , Janeschik, Matthias (Author) , Schaub, Christoph (Author) , Lohmann, Ingrid (Author)
Format: Article (Journal)
Language:English
Published: 5 January 2021
In: Cell reports
Year: 2021, Volume: 34, Issue: 1
ISSN:2211-1247
DOI:10.1016/j.celrep.2020.108577
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.celrep.2020.108577
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S2211124720315667
Get full text
Author Notes:Katrin Domsch, Julia Schröder, Matthias Janeschik, Christoph Schaub, Ingrid Lohmann

MARC

LEADER 00000caa a2200000 c 4500
001 1749161982
003 DE-627
005 20240411193435.0
007 cr uuu---uuuuu
008 210223s2021 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.celrep.2020.108577  |2 doi 
035 |a (DE-627)1749161982 
035 |a (DE-599)KXP1749161982 
035 |a (OCoLC)1341395497 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Domsch, Katrin  |e VerfasserIn  |0 (DE-588)1135800960  |0 (DE-627)890881103  |0 (DE-576)490137989  |4 aut 
245 1 4 |a The Hox transcription factor Ubx ensures somatic myogenesis by suppressing the mesodermal master regulator Twist  |c Katrin Domsch, Julia Schröder, Matthias Janeschik, Christoph Schaub, Ingrid Lohmann 
264 1 |c 5 January 2021 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 23.02.2021 
520 |a Early lineage-specific master regulators are essential for the specification of cell types. However, once cells are committed to a specific fate, it is critical to restrict the activity of such factors to enable differentiation. To date, it remains unclear how these factors are silenced. Using the Drosophila mesoderm as a model and a comparative genomic approach, we identify the Hox transcription factor Ultrabithorax (Ubx) to be critical for the repression of the master regulator Twist. Mesoderm-specific Ubx loss-of-function experiments using CRISPR-Cas9 and overexpression studies demonstrate that Ubx majorly impacts twist transcription. A mechanistic analysis reveals that Ubx requires the NK-homeodomain protein Tinman to bind to the twist promoter. Furthermore, we find these factor interactions to be critical for silencing by recruiting the Polycomb DNA binding protein Pleiohomeotic. Altogether, our data reveal that Ubx is a critical player in mediating the silencing of Twist, which is crucial for coordinated muscle differentiation. 
650 4 |a Hox genes 
650 4 |a Mef2 
650 4 |a mesoderm 
650 4 |a repression 
650 4 |a Tinman 
650 4 |a twist 
650 4 |a Ultrabithorax (Ubx) 
700 1 |a Schröder, Julia  |e VerfasserIn  |4 aut 
700 1 |a Janeschik, Matthias  |e VerfasserIn  |4 aut 
700 1 |a Schaub, Christoph  |e VerfasserIn  |4 aut 
700 1 |a Lohmann, Ingrid  |e VerfasserIn  |0 (DE-588)1047761831  |0 (DE-627)779177215  |0 (DE-576)401571688  |4 aut 
773 0 8 |i Enthalten in  |t Cell reports  |d Maryland Heights, MO : Cell Press, 2012  |g 34(2021,1) Artikel-Nummer 108577,14 Seiten  |h Online-Ressource  |w (DE-627)684964562  |w (DE-600)2649101-1  |w (DE-576)358411572  |x 2211-1247  |7 nnas  |a The Hox transcription factor Ubx ensures somatic myogenesis by suppressing the mesodermal master regulator Twist 
773 1 8 |g volume:34  |g year:2021  |g number:1  |a The Hox transcription factor Ubx ensures somatic myogenesis by suppressing the mesodermal master regulator Twist 
856 4 0 |u https://doi.org/10.1016/j.celrep.2020.108577  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.sciencedirect.com/science/article/pii/S2211124720315667  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20210223 
993 |a Article 
994 |a 2021 
998 |g 1047761831  |a Lohmann, Ingrid  |m 1047761831:Lohmann, Ingrid  |d 700000  |d 721000  |e 700000PL1047761831  |e 721000PL1047761831  |k 0/700000/  |k 1/700000/721000/  |p 5  |y j 
998 |g 1135800960  |a Domsch, Katrin  |m 1135800960:Domsch, Katrin  |p 1  |x j 
999 |a KXP-PPN1749161982  |e 387081621X 
BIB |a Y 
SER |a journal 
JSO |a {"title":[{"title_sort":"Hox transcription factor Ubx ensures somatic myogenesis by suppressing the mesodermal master regulator Twist","title":"The Hox transcription factor Ubx ensures somatic myogenesis by suppressing the mesodermal master regulator Twist"}],"id":{"eki":["1749161982"],"doi":["10.1016/j.celrep.2020.108577"]},"relHost":[{"pubHistory":["1.2012 -"],"type":{"bibl":"periodical","media":"Online-Ressource"},"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"part":{"text":"34(2021,1) Artikel-Nummer 108577,14 Seiten","volume":"34","year":"2021","issue":"1"},"disp":"The Hox transcription factor Ubx ensures somatic myogenesis by suppressing the mesodermal master regulator TwistCell reports","recId":"684964562","origin":[{"dateIssuedDisp":"2012-","dateIssuedKey":"2012","publisherPlace":"Maryland Heights, MO ; [New York, NY]","publisher":"Cell Press ; Elsevier"}],"id":{"issn":["2211-1247"],"zdb":["2649101-1"],"eki":["684964562"]},"title":[{"title":"Cell reports","title_sort":"Cell reports"}]}],"recId":"1749161982","origin":[{"dateIssuedDisp":"5 January 2021","dateIssuedKey":"2021"}],"language":["eng"],"note":["Gesehen am 23.02.2021"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["Katrin Domsch, Julia Schröder, Matthias Janeschik, Christoph Schaub, Ingrid Lohmann"]},"person":[{"family":"Domsch","given":"Katrin","roleDisplay":"VerfasserIn","role":"aut","display":"Domsch, Katrin"},{"roleDisplay":"VerfasserIn","display":"Schröder, Julia","role":"aut","family":"Schröder","given":"Julia"},{"family":"Janeschik","given":"Matthias","role":"aut","display":"Janeschik, Matthias","roleDisplay":"VerfasserIn"},{"given":"Christoph","family":"Schaub","roleDisplay":"VerfasserIn","role":"aut","display":"Schaub, Christoph"},{"given":"Ingrid","family":"Lohmann","roleDisplay":"VerfasserIn","display":"Lohmann, Ingrid","role":"aut"}]} 
SRT |a DOMSCHKATRHOXTRANSCR5202