Electron tomography of plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion
Erythrocyte invasion by merozoites forms of the malaria parasite is a key step in the establishment of human malaria disease. To date, efforts to understand cellular events underpinning entry have been limited to insights from non-human parasites, with no studies at sub-micrometer resolution underta...
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| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
22 March 2013
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| In: |
Cellular microbiology
Year: 2013, Jahrgang: 15, Heft: 9, Pages: 1457-1472 |
| ISSN: | 1462-5822 |
| DOI: | https://doi.org/10.1111/cmi.12132 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1111/cmi.12132 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.12132 
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| Verfasserangaben: | Eric Hanssen, Chaitali Dekiwadia, David T. Riglar, Melanie Rug, Leandro Lemgruber, Alan F. Cowman, Marek Cyrklaff, Mikhail Kudryashev, Friedrich Frischknecht, Jake Baum and Stuart A. Ralph |
| Zusammenfassung: | Erythrocyte invasion by merozoites forms of the malaria parasite is a key step in the establishment of human malaria disease. To date, efforts to understand cellular events underpinning entry have been limited to insights from non-human parasites, with no studies at sub-micrometer resolution undertaken using the most virulent human malaria parasite, Plasmodium falciparum. This leaves our understanding of the dynamics of merozoite sub-cellular compartments during infectionincomplete, in particular that of the secretory organelles. Using advances in P. falciparum merozoite isolation and new imaging techniques we present a three-dimensional study of invasion using electron microscopy, cryo-electron tomography and cryo-X-ray tomography. We describe the core architectural features of invasion and identify fusion between rhoptries at the commencement of invasion as a hitherto overlooked event that likely provides a critical step that initiates entry. Given the centrality of merozoite organelle proteins to vaccine development, these insights provide a mechanistic framework to understand therapeutic strategies targeted towards the cellular events of invasion. |
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| Beschreibung: | Gesehen am 26.02.2021 |
| Beschreibung: | Online Resource |
| ISSN: | 1462-5822 |
| DOI: | https://doi.org/10.1111/cmi.12132 |