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Phenotype and function of antigen-presenting dendritic cells generated from peripheral blood monocytes

Background: Dendritic cells (DC) are specialized for the primary activation of helper and cytotoxic T cells and have therefore been denominated ‘professional’ antigen-presenting cells. Their potent antigen-presenting capacity qualifies these cells as a promising tool in vaccination protocols, in par...

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Bibliographic Details
Main Authors: Bauer, Yvonne (Author) , Jäger, Claudia (Author) , Kramer, Michael D. (Author) , Wallich, Reinhard (Author)
Format: Article (Journal)
Language:English
Published: 1999
In: Transfusion medicine and hemotherapy
Year: 1999, Volume: 26, Issue: 2, Pages: 115-118
ISSN:1660-3818
DOI:10.1159/000053474
Online Access:Verlag, lizenzpflichtig, Volltext: https://dx.doi.org/10.1159/000053474
Verlag, lizenzpflichtig, Volltext: https://www.karger.com/Article/FullText/53474
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Author Notes:Y. Bauer, C. Jäger, M.D. Kramer, R. Wallich
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Summary:Background: Dendritic cells (DC) are specialized for the primary activation of helper and cytotoxic T cells and have therefore been denominated ‘professional’ antigen-presenting cells. Their potent antigen-presenting capacity qualifies these cells as a promising tool in vaccination protocols, in particular in the induction of tumor-specific immunity. Functionally active DC can be generated from peripheral blood monocytes in vitro. Consequently, these cells gained interest in the field of transfusion medicine. Material and Methods: Here we describe a simplified protocol for the generation of large numbers of DC from peripheral blood monocytes and their phenotype and function. DC were generated from CD14+ monocytes by culture in the presence of granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). Results: On day 7 of culture the expression of the surface markers CD1a, CD80 and HLA-DR was upregulated, whereas CD14 and CD64 were almost completely downregulated, compared to day 0. Moreover, the in vitro generated DC were shown to induce antigen-specific proliferation of autologous T cells after pulsing with tetanus toxoid. Conclusion: The presented protocol allows to generate large numbers of DC for immunotherapy and vaccination.
Item Description:Gesehen am 01.03.2021
Physical Description:Online Resource
ISSN:1660-3818
DOI:10.1159/000053474

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