Phenotype and function of antigen-presenting dendritic cells generated from peripheral blood monocytes
Background: Dendritic cells (DC) are specialized for the primary activation of helper and cytotoxic T cells and have therefore been denominated ‘professional’ antigen-presenting cells. Their potent antigen-presenting capacity qualifies these cells as a promising tool in vaccination protocols, in par...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
1999
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| In: |
Transfusion medicine and hemotherapy
Year: 1999, Volume: 26, Issue: 2, Pages: 115-118 |
| ISSN: | 1660-3818 |
| DOI: | 10.1159/000053474 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://dx.doi.org/10.1159/000053474 Verlag, lizenzpflichtig, Volltext: https://www.karger.com/Article/FullText/53474 
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| Author Notes: | Y. Bauer, C. Jäger, M.D. Kramer, R. Wallich |
MARC
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| 245 | 1 | 0 | |a Phenotype and function of antigen-presenting dendritic cells generated from peripheral blood monocytes |c Y. Bauer, C. Jäger, M.D. Kramer, R. Wallich |
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| 520 | |a Background: Dendritic cells (DC) are specialized for the primary activation of helper and cytotoxic T cells and have therefore been denominated ‘professional’ antigen-presenting cells. Their potent antigen-presenting capacity qualifies these cells as a promising tool in vaccination protocols, in particular in the induction of tumor-specific immunity. Functionally active DC can be generated from peripheral blood monocytes in vitro. Consequently, these cells gained interest in the field of transfusion medicine. Material and Methods: Here we describe a simplified protocol for the generation of large numbers of DC from peripheral blood monocytes and their phenotype and function. DC were generated from CD14+ monocytes by culture in the presence of granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). Results: On day 7 of culture the expression of the surface markers CD1a, CD80 and HLA-DR was upregulated, whereas CD14 and CD64 were almost completely downregulated, compared to day 0. Moreover, the in vitro generated DC were shown to induce antigen-specific proliferation of autologous T cells after pulsing with tetanus toxoid. Conclusion: The presented protocol allows to generate large numbers of DC for immunotherapy and vaccination. | ||
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