Enhancement of Sia-lb1 antigenicity by sulphur-containing substituents at C-5 of free N-acetylneuraminic acid

The Sia-lb1 epitope, recognized by anti-Sia-lb1 cold agglutinins, is unique since it is represented by the alpha-N-acetylneuraminic acid (alpha NeuNAc) monosaccharide. Chemical modifications of the chain at C-5 of alpha NeuNAc have shown that the natural 2-carbon and the artificial 3-carbon chains a...

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Hauptverfasser: Roelcke, Dieter (VerfasserIn) , Leo, Albrecht (VerfasserIn) , Brossmer, Reinhard (VerfasserIn)
Dokumenttyp: Article (Journal) Kapitel/Artikel
Sprache:Englisch
Veröffentlicht: 1997
In: Beiträge zur Infusionstherapie und Transfusionsmedizin
Year: 1997, Jahrgang: 34, Pages: 215-219
Online-Zugang: Volltext
Verfasserangaben:D. Roelcke, A. Leo, R. Brossmer

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520 |a The Sia-lb1 epitope, recognized by anti-Sia-lb1 cold agglutinins, is unique since it is represented by the alpha-N-acetylneuraminic acid (alpha NeuNAc) monosaccharide. Chemical modifications of the chain at C-5 of alpha NeuNAc have shown that the natural 2-carbon and the artificial 3-carbon chains are optimal for anti-Sia-lb1 binding. Sia-lb1 antigenicity of alpha NeuNAc could be tenfold enhanced by replacement of the carbonyl oxygen by sulphur. The structural requirements of the Sia-lb1 epitope for optimal antibody binding were identified. 
650 4 |a Agglutinins 
650 4 |a Amino Acid Substitution 
650 4 |a Amino Acids, Sulfur 
650 4 |a Binding Sites, Antibody 
650 4 |a Cryoglobulins 
650 4 |a Epitopes 
650 4 |a Humans 
650 4 |a Isoantigens 
650 4 |a N-Acetylneuraminic Acid 
650 4 |a Structure-Activity Relationship 
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