Production of antibodies to the constant region of the mouse T-cell-receptor β-chain
To generate antibodies to the constant region of the mouse T-cell-receptor β chain, the corresponding cDNA clone 86T5 was subcloned into the bacterial expression vector pEX2. The induced hybrid protein consisting of a 30 kDa T-cell-receptor segment and a β-galactosidase carrier moiety was used for i...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
1991
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| In: |
Acta pathologica, microbiologica et immunologica Scandinavica
Year: 1991, Volume: 99, Issue: 1-6, Pages: 99-102 |
| ISSN: | 1600-0463 |
| DOI: | https://doi.org/10.1111/j.1699-0463.1991.tb05126.x |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://dx.doi.org/https://doi.org/10.1111/j.1699-0463.1991.tb05126.x Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1699-0463.1991.tb05126.x |
| Author Notes: | Johannes Schenkel, Hubert Schäfer, Susanne Koch, Reinhard Burger |
MARC
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| 520 | |a To generate antibodies to the constant region of the mouse T-cell-receptor β chain, the corresponding cDNA clone 86T5 was subcloned into the bacterial expression vector pEX2. The induced hybrid protein consisting of a 30 kDa T-cell-receptor segment and a β-galactosidase carrier moiety was used for immunization. In Western blots using lysates of a T-cell-receptor-positive T-cell hybrid, the antisera obtained reacted with a 42 kDa protein under reducing and a 85 kDa protein under nonreducing conditions. The antisera did not react in binding assays with intact T lymphocytes, i.e. membrane-associated T-cell-receptor protein. | ||
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