Fibulin-2: a novel biomarker for differentiating grade II from grade I meningiomas
There is an unmet need for the identification of biomarkers to aid in the diagnosis, clinical management, prognosis and follow-up of meningiomas. There is currently no consensus on the optimum management of WHO grade II meningiomas. In this study, we identified the calcium binding extracellular matr...
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| Hauptverfasser: | , , , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
8 January 2021
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| In: |
International journal of molecular sciences
Year: 2021, Jahrgang: 22, Heft: 2, Pages: 1-13 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22020560 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/ijms22020560 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/1422-0067/22/2/560 
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| Verfasserangaben: | Agbolahan A. Sofela, David A. Hilton, Sylwia Ammoun, Daniele Baiz, Claire L. Adams, Emanuela Ercolano, Michael D. Jenkinson, Kathreena M. Kurian, Mario Teo, Peter C. Whitfield, Felix Sahm and C. Oliver Hanemann |
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| 245 | 1 | 0 | |a Fibulin-2 |b a novel biomarker for differentiating grade II from grade I meningiomas |c Agbolahan A. Sofela, David A. Hilton, Sylwia Ammoun, Daniele Baiz, Claire L. Adams, Emanuela Ercolano, Michael D. Jenkinson, Kathreena M. Kurian, Mario Teo, Peter C. Whitfield, Felix Sahm and C. Oliver Hanemann |
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| 520 | |a There is an unmet need for the identification of biomarkers to aid in the diagnosis, clinical management, prognosis and follow-up of meningiomas. There is currently no consensus on the optimum management of WHO grade II meningiomas. In this study, we identified the calcium binding extracellular matrix glycoprotein, Fibulin-2, via mass-spectrometry-based proteomics, assessed its expression in grade I and II meningiomas and explored its potential as a grade II biomarker. A total of 87 grade I and 91 grade II different meningioma cells, tissue and plasma samples were used for the various experimental techniques employed to assess Fibulin-2 expression. The tumours were reviewed and classified according to the 2016 edition of the Classification of the Tumours of the central nervous system (CNS). Mass spectrometry proteomic analysis identified Fibulin-2 as a differentially expressed protein between grade I and II meningioma cell cultures. Fibulin-2 levels were further evaluated in meningioma cells using Western blotting and Real-time Quantitative Polymerase Chain Reaction (RT-qPCR); in meningioma tissues via immunohistochemistry and RT-qPCR; and in plasma via Enzyme-Linked Immunosorbent Assay (ELISA). Proteomic analyses (p < 0.05), Western blotting (p < 0.05) and RT-qPCR (p < 0.01) confirmed significantly higher Fibulin-2 (FBLN2) expression levels in grade II meningiomas compared to grade I. Fibulin-2 blood plasma levels were also significantly higher in grade II meningioma patients compared to grade I patients. This study suggests that elevated Fibulin-2 might be a novel grade II meningioma biomarker, when differentiating them from the grade I tumours. The trend of Fibulin-2 expression observed in plasma may serve as a useful non-invasive biomarker. | ||
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| 700 | 1 | |a Hilton, David A. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ammoun, Sylwia |e VerfasserIn |4 aut | |
| 700 | 1 | |a Baiz, Daniele |e VerfasserIn |4 aut | |
| 700 | 1 | |a Adams, Claire L. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ercolano, Emanuela |e VerfasserIn |4 aut | |
| 700 | 1 | |a Jenkinson, Michael D. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kurian, Kathreena M. |e VerfasserIn |4 aut | |
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| 700 | 1 | |a Hanemann, C. Oliver |e VerfasserIn |4 aut | |
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