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Recognition of defined epitopes by affinity-purified anti-immunoglobulin fab autoantibodies isolated from HIV-infected humans

Infection of humans with HIV-1 has previously been independently shown to result in the generation of autoantibodies (AAbs) reactive with immunoglobulin Fab fragments (Heidelberg), and with autoantibodies to T-cell receptors (TCRs) (Tucson). Here, we carry out epitope mapping studies of affinity-pur...

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Hauptverfasser: Marchalonis, John J. (VerfasserIn) , Garza, A. (VerfasserIn) , Lake, D. F. (VerfasserIn) , Landsperger, W. J. (VerfasserIn) , Süsal, Caner (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 1999
In: Journal of molecular recognition
Year: 1999, Jahrgang: 12, Heft: 3, Pages: 169-176
ISSN:1099-1352
DOI:10.1002/(SICI)1099-1352(199905/06)12:3<169::AID-JMR459>3.0.CO;2-6
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/(SICI)1099-1352(199905/06)12:3<169::AID-JMR459>3.0.CO;2-6
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/10.1002/(SICI)1099-1352(199905/06)12:3%3C169::AID-JMR459%3E3.0.CO;2-6
Volltext
Verfasserangaben:John J. Marchalonis, Andrea Garza, Douglas F. Lake, William J. Landsperger, Caner Süsal

MARC

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245 1 0 |a Recognition of defined epitopes by affinity-purified anti-immunoglobulin fab autoantibodies isolated from HIV-infected humans  |c John J. Marchalonis, Andrea Garza, Douglas F. Lake, William J. Landsperger, Caner Süsal 
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520 |a Infection of humans with HIV-1 has previously been independently shown to result in the generation of autoantibodies (AAbs) reactive with immunoglobulin Fab fragments (Heidelberg), and with autoantibodies to T-cell receptors (TCRs) (Tucson). Here, we carry out epitope mapping studies of affinity-purified AAbs to Fab fragments prepared from individual HIV-positive patients for their capacity to bind recombinant constructs and peptide-defined epitopes modeling TCR and Ig light chains. Some affinity-purified autoantibodies reacted strongly with TCRs expressed by intact T-cells, and recombinant Valpha/Vbeta constructs as well as with certain synthetic peptide epitopes. The binding reactions of affinity-purified AAbs of individual patients were distinct, and the AAb preparations consisted of populations of polyclonal lgs as reflected in specificity and isotype. AAb pools from individual patients all bound particular regions of TCR and Ig chains defined by comprehensive peptide synthesis including the CDR1 and Fr3 segments of the variable domains and the joining segment/switch peptide. In addition, other reactivities to restricted regions of alpha, beta and lambda light chains were documented. These results substantiate the cross-reactivity of TCR and Ig-Fab determinants, and are consistent with the hypothesis that autoantibodies arising as a consequence of HIV infection can have an immunomodulatory role. 
650 4 |a Amino Acid Sequence 
650 4 |a Antibodies, Anti-Idiotypic 
650 4 |a Antibody Affinity 
650 4 |a Antigen-Antibody Reactions 
650 4 |a Autoantibodies 
650 4 |a Autoantigens 
650 4 |a Cross Reactions 
650 4 |a Enzyme-Linked Immunosorbent Assay 
650 4 |a Epitopes 
650 4 |a HIV Infections 
650 4 |a HIV-1 
650 4 |a Humans 
650 4 |a Immunoglobulin Fab Fragments 
650 4 |a Molecular Sequence Data 
650 4 |a Peptide Fragments 
650 4 |a Receptors, Antigen, T-Cell, alpha-beta 
650 4 |a Recombinant Fusion Proteins 
650 4 |a Sequence Alignment 
650 4 |a Sequence Homology, Amino Acid 
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700 1 |a Landsperger, W. J.  |e VerfasserIn  |4 aut 
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