Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
01 February 2021
|
| In: |
Communications biology
Year: 2021, Volume: 4, Pages: 1-10 |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-021-01661-w |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s42003-021-01661-w Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s42003-021-01661-w 
|
| Author Notes: | Christoph Niemöller, Julius Wehrle, Julian Riba, Rainer Claus, Nathalie Renz, Janika Rhein, Sabine Bleul, Juliane M. Stosch, Justus Duyster, Christoph Plass, Pavlo Lutsik, Daniel B. Lipka, Michael Lübbert & Heiko Becker |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1751172015 | ||
| 003 | DE-627 | ||
| 005 | 20240412193302.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210312s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s42003-021-01661-w |2 doi | |
| 035 | |a (DE-627)1751172015 | ||
| 035 | |a (DE-599)KXP1751172015 | ||
| 035 | |a (OCoLC)1341398486 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Niemöller, Christoph |e VerfasserIn |0 (DE-588)1246549816 |0 (DE-627)177960808X |4 aut | |
| 245 | 1 | 0 | |a Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction |c Christoph Niemöller, Julius Wehrle, Julian Riba, Rainer Claus, Nathalie Renz, Janika Rhein, Sabine Bleul, Juliane M. Stosch, Justus Duyster, Christoph Plass, Pavlo Lutsik, Daniel B. Lipka, Michael Lübbert & Heiko Becker |
| 264 | 1 | |c 01 February 2021 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 26.11.2021 | ||
| 520 | |a Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles. | ||
| 700 | 1 | |a Plass, Christoph |d 1961- |e VerfasserIn |0 (DE-588)116753347X |0 (DE-627)1031159894 |0 (DE-576)51118106X |4 aut | |
| 700 | 1 | |a Lutsik, Pavlo |e VerfasserIn |0 (DE-588)1176218166 |0 (DE-627)1047372894 |0 (DE-576)516581988 |4 aut | |
| 700 | 1 | |a Lipka, Daniel |d 1976- |e VerfasserIn |0 (DE-588)131915312 |0 (DE-627)516076426 |0 (DE-576)298833328 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Communications biology |d London : Springer Nature, 2018 |g 4(2021), Artikel-ID 153, Seite 1-10 |h Online-Ressource |w (DE-627)101369080X |w (DE-600)2919698-X |w (DE-576)499739132 |x 2399-3642 |7 nnas |a Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction |
| 773 | 1 | 8 | |g volume:4 |g year:2021 |g elocationid:153 |g pages:1-10 |g extent:10 |a Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s42003-021-01661-w |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s42003-021-01661-w |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210312 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 131915312 |a Lipka, Daniel |m 131915312:Lipka, Daniel |d 140000 |e 140000PL131915312 |k 0/140000/ |p 12 | ||
| 998 | |g 1176218166 |a Lutsik, Pavlo |m 1176218166:Lutsik, Pavlo |d 140000 |e 140000PL1176218166 |k 0/140000/ |p 11 | ||
| 998 | |g 116753347X |a Plass, Christoph |m 116753347X:Plass, Christoph |d 50000 |e 50000PP116753347X |k 0/50000/ |p 10 | ||
| 999 | |a KXP-PPN1751172015 |e 3885731193 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"id":{"eki":["101369080X"],"zdb":["2919698-X"],"issn":["2399-3642"]},"origin":[{"dateIssuedDisp":"[2018]-","publisher":"Springer Nature","publisherPlace":"London"}],"part":{"year":"2021","text":"4(2021), Artikel-ID 153, Seite 1-10","extent":"10","volume":"4","pages":"1-10"},"recId":"101369080X","disp":"Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restrictionCommunications biology","title":[{"title":"Communications biology","title_sort":"Communications biology"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"physDesc":[{"extent":"Online-Ressource"}],"pubHistory":["1 (2018)-"],"language":["eng"]}],"note":["Gesehen am 26.11.2021"],"id":{"eki":["1751172015"],"doi":["10.1038/s42003-021-01661-w"]},"origin":[{"dateIssuedKey":"2021","dateIssuedDisp":"01 February 2021"}],"name":{"displayForm":["Christoph Niemöller, Julius Wehrle, Julian Riba, Rainer Claus, Nathalie Renz, Janika Rhein, Sabine Bleul, Juliane M. Stosch, Justus Duyster, Christoph Plass, Pavlo Lutsik, Daniel B. Lipka, Michael Lübbert & Heiko Becker"]},"recId":"1751172015","person":[{"given":"Christoph","role":"aut","family":"Niemöller","display":"Niemöller, Christoph"},{"display":"Plass, Christoph","family":"Plass","role":"aut","given":"Christoph"},{"display":"Lutsik, Pavlo","family":"Lutsik","given":"Pavlo","role":"aut"},{"display":"Lipka, Daniel","role":"aut","given":"Daniel","family":"Lipka"}],"title":[{"title_sort":"Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction","title":"Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"extent":"10 S."}],"language":["eng"]} | ||
| SRT | |a NIEMOELLERBISULFITEF0120 | ||