Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
01 February 2021
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| In: |
Communications biology
Year: 2021, Volume: 4, Pages: 1-10 |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-021-01661-w |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s42003-021-01661-w Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s42003-021-01661-w 
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| Author Notes: | Christoph Niemöller, Julius Wehrle, Julian Riba, Rainer Claus, Nathalie Renz, Janika Rhein, Sabine Bleul, Juliane M. Stosch, Justus Duyster, Christoph Plass, Pavlo Lutsik, Daniel B. Lipka, Michael Lübbert & Heiko Becker |
| Summary: | Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles. |
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| Item Description: | Gesehen am 26.11.2021 |
| Physical Description: | Online Resource |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-021-01661-w |