Rescue of silenced UCHL1 and IGFBP4 expression suppresses clonogenicity of giant cell tumor-derived stromal cells
Giant cell tumor (GCT) of bone is a generally benign tumor with a locally aggressive behavior. Histologically, GCTs consist of multinucleated giant cells, mononuclear histiocytes and the neoplastic fibroblast-like stromal cells (GCTSC). Growing evidence exists that GCTSCs develop from mesenchymal st...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
9 August 2013
|
| In: |
Cancer letters
Year: 2013, Jahrgang: 336, Heft: 1, Pages: 61-67 |
| ISSN: | 1872-7980 |
| DOI: | 10.1016/j.canlet.2013.04.011 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.canlet.2013.04.011 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0304383513003315 
|
| Verfasserangaben: | Jörg Fellenberg, Heiner Sähr, Li Liu, Frank Schönsiegel, Daniela Depeweg, Burkhard Lehner, Ingrid Herr |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1751207706 | ||
| 003 | DE-627 | ||
| 005 | 20220819134311.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210312s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.canlet.2013.04.011 |2 doi | |
| 035 | |a (DE-627)1751207706 | ||
| 035 | |a (DE-599)KXP1751207706 | ||
| 035 | |a (OCoLC)1341398597 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Fellenberg, Jörg |e VerfasserIn |0 (DE-588)1063323932 |0 (DE-627)812309219 |0 (DE-576)171796543 |4 aut | |
| 245 | 1 | 0 | |a Rescue of silenced UCHL1 and IGFBP4 expression suppresses clonogenicity of giant cell tumor-derived stromal cells |c Jörg Fellenberg, Heiner Sähr, Li Liu, Frank Schönsiegel, Daniela Depeweg, Burkhard Lehner, Ingrid Herr |
| 264 | 1 | |c 9 August 2013 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 12.03.2021 | ||
| 520 | |a Giant cell tumor (GCT) of bone is a generally benign tumor with a locally aggressive behavior. Histologically, GCTs consist of multinucleated giant cells, mononuclear histiocytes and the neoplastic fibroblast-like stromal cells (GCTSC). Growing evidence exists that GCTSCs develop from mesenchymal stem cells (MSCs), but little is known about the underlying molecular mechanisms. In previous studies we observed inactivation of the ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) gene in primary GCTSC due to strong DNA hypermethylation, indicating that epigenetic silencing might be involved in neoplastic transformation of MSCs. Here we investigated further candidate genes and identified strong hypermethylation of the insulin-like growth factor binding protein 4 (IGFBP4) promoter, resulting in IGFBP4 downregulation in GCTs compared to MSCs. Overexpression of UCHL1 and IGFBP4 by stable transfection of GCTSC did not influence cell viability, proliferation, migration and chemosensitivity compared to parental cells. However, colony-formation was significantly decreased suggesting that rescue of UCHL1 and IFGBP4 suppresses clonogenicity of GCT stromal cells. The observation of reduced expression of the stem-cell-specific transcription factors OCT4 and SOX2 in these cell lines further supported our findings. Epigenetic silencing of UCHL1 and IGFBP4 in GCTs might thus be a crucial event during the malignant transformation of MSCs in the context of GCT development and represent promising targets for the development of new diagnostic and therapeutic strategies. | ||
| 650 | 4 | |a Epigenetic silencing | |
| 650 | 4 | |a Giant cell tumor | |
| 650 | 4 | |a IGFBP4 | |
| 650 | 4 | |a Mesenchymal stem cell | |
| 650 | 4 | |a Promoter hypermethylation | |
| 650 | 4 | |a UCHL1 | |
| 700 | 1 | |a Sähr, Heiner |e VerfasserIn |0 (DE-588)1063323940 |0 (DE-627)812309316 |0 (DE-576)42184650X |4 aut | |
| 700 | 1 | |a Liu, Li |d 1979- |e VerfasserIn |0 (DE-588)1025450906 |0 (DE-627)722293267 |0 (DE-576)370359291 |4 aut | |
| 700 | 1 | |a Schönsiegel, Frank |d 1972- |e VerfasserIn |0 (DE-588)129162159 |0 (DE-627)390028991 |0 (DE-576)297516051 |4 aut | |
| 700 | 1 | |a Depeweg, Daniela |e VerfasserIn |0 (DE-588)1072835673 |0 (DE-627)82854042X |0 (DE-576)434279161 |4 aut | |
| 700 | 1 | |a Lehner, Burkhard |e VerfasserIn |0 (DE-588)1063147808 |0 (DE-627)810647311 |0 (DE-576)420525297 |4 aut | |
| 700 | 1 | |a Herr, Ingrid |e VerfasserIn |0 (DE-588)114884242 |0 (DE-627)691233276 |0 (DE-576)168702436 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cancer letters |d Amsterdam [u.a.] : Elsevier Science, 1975 |g 336(2013), 1, Seite 61-67 |h Online-Ressource |w (DE-627)320434796 |w (DE-600)2004212-7 |w (DE-576)261677926 |x 1872-7980 |7 nnas |a Rescue of silenced UCHL1 and IGFBP4 expression suppresses clonogenicity of giant cell tumor-derived stromal cells |
| 773 | 1 | 8 | |g volume:336 |g year:2013 |g number:1 |g pages:61-67 |g extent:7 |a Rescue of silenced UCHL1 and IGFBP4 expression suppresses clonogenicity of giant cell tumor-derived stromal cells |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.canlet.2013.04.011 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0304383513003315 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210312 | ||
| 993 | |a Article | ||
| 994 | |a 2013 | ||
| 998 | |g 114884242 |a Herr, Ingrid |m 114884242:Herr, Ingrid |d 910000 |d 910200 |e 910000PH114884242 |e 910200PH114884242 |k 0/910000/ |k 1/910000/910200/ |p 7 |y j | ||
| 998 | |g 1063147808 |a Lehner, Burkhard |m 1063147808:Lehner, Burkhard |d 910000 |d 911460 |e 910000PL1063147808 |e 911460PL1063147808 |k 0/910000/ |k 1/910000/911460/ |p 6 | ||
| 998 | |g 1072835673 |a Depeweg, Daniela |m 1072835673:Depeweg, Daniela |d 910000 |d 911460 |e 910000PD1072835673 |e 911460PD1072835673 |k 0/910000/ |k 1/910000/911460/ |p 5 | ||
| 998 | |g 129162159 |a Schönsiegel, Frank |m 129162159:Schönsiegel, Frank |d 140000 |e 140000PS129162159 |k 0/140000/ |p 4 | ||
| 998 | |g 1025450906 |a Liu, Li |m 1025450906:Liu, Li |d 50000 |e 50000PL1025450906 |k 0/50000/ |p 3 | ||
| 998 | |g 1063323940 |a Sähr, Heiner |m 1063323940:Sähr, Heiner |d 50000 |e 50000PS1063323940 |k 0/50000/ |p 2 | ||
| 998 | |g 1063323932 |a Fellenberg, Jörg |m 1063323932:Fellenberg, Jörg |d 910000 |d 911460 |e 910000PF1063323932 |e 911460PF1063323932 |k 0/910000/ |k 1/910000/911460/ |p 1 |x j | ||
| 999 | |a KXP-PPN1751207706 |e 3885850974 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1751207706","relHost":[{"pubHistory":["1.1975 -"],"note":["Gesehen am 22.01.2020"],"origin":[{"publisher":"Elsevier Science","dateIssuedDisp":"1975-","dateIssuedKey":"1975","publisherPlace":"Amsterdam [u.a.]"}],"recId":"320434796","language":["eng"],"id":{"issn":["1872-7980"],"eki":["320434796"],"zdb":["2004212-7"]},"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"Rescue of silenced UCHL1 and IGFBP4 expression suppresses clonogenicity of giant cell tumor-derived stromal cellsCancer letters","physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Cancer letters","subtitle":"an international journal providing a forum for original and pertinent contributions in cancer research","title":"Cancer letters"}],"part":{"issue":"1","extent":"7","volume":"336","text":"336(2013), 1, Seite 61-67","pages":"61-67","year":"2013"}}],"note":["Gesehen am 12.03.2021"],"origin":[{"dateIssuedKey":"2013","dateIssuedDisp":"9 August 2013"}],"person":[{"family":"Fellenberg","display":"Fellenberg, Jörg","given":"Jörg","role":"aut"},{"display":"Sähr, Heiner","given":"Heiner","role":"aut","family":"Sähr"},{"role":"aut","given":"Li","display":"Liu, Li","family":"Liu"},{"display":"Schönsiegel, Frank","given":"Frank","role":"aut","family":"Schönsiegel"},{"given":"Daniela","display":"Depeweg, Daniela","role":"aut","family":"Depeweg"},{"display":"Lehner, Burkhard","given":"Burkhard","role":"aut","family":"Lehner"},{"role":"aut","display":"Herr, Ingrid","given":"Ingrid","family":"Herr"}],"title":[{"title_sort":"Rescue of silenced UCHL1 and IGFBP4 expression suppresses clonogenicity of giant cell tumor-derived stromal cells","title":"Rescue of silenced UCHL1 and IGFBP4 expression suppresses clonogenicity of giant cell tumor-derived stromal cells"}],"physDesc":[{"extent":"7 S."}],"id":{"eki":["1751207706"],"doi":["10.1016/j.canlet.2013.04.011"]},"name":{"displayForm":["Jörg Fellenberg, Heiner Sähr, Li Liu, Frank Schönsiegel, Daniela Depeweg, Burkhard Lehner, Ingrid Herr"]},"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"}} | ||
| SRT | |a FELLENBERGRESCUEOFSI9201 | ||