Detection of inducible nitric oxide synthase in Schistosoma japonicum and S. mansoni
Schistosoma japonicum and S. mansoni were tested for reactivity with an anti-inducible nitric oxide (iNOS) antibody and the distribution of iNOS was studied by immunofluorescent tests in different stages of the parasites. Reactivity was associated with the tegument in both larval schistosomes (sporo...
Gespeichert in:
| Hauptverfasser: | , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2004
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| In: |
Journal of helminthology
Year: 2004, Jahrgang: 78, Heft: 1, Pages: 47-50 |
| ISSN: | 1475-2697 |
| DOI: | 10.1079/joh2003202 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1079/joh2003202
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| Verfasserangaben: | X.-C. Long, M. Bahgat, K. Chlichlia, A. Ruppel and Y.-L. Li |
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| 520 | |a Schistosoma japonicum and S. mansoni were tested for reactivity with an anti-inducible nitric oxide (iNOS) antibody and the distribution of iNOS was studied by immunofluorescent tests in different stages of the parasites. Reactivity was associated with the tegument in both larval schistosomes (sporocysts and cercariae) and eggs. With adult worms, the majority of the immunofluorescence was predominantly subtegumental in S. japonicum and parenchymal in S. mansoni. Fluorescence was also observed in host tissues (snails and mouse liver). In Western blots, the enzyme of S. japonicum had an apparent molecular weight of about 210 kDa. The possible role of worm and host iNOS in the parasite-host interrelation remains to be clarified. | ||
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| 650 | 4 | |a Nitric Oxide Synthase | |
| 650 | 4 | |a Nitric Oxide Synthase Type II | |
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| 650 | 4 | |a Schistosoma mansoni | |
| 650 | 4 | |a Schistosomiasis | |
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