Hiding in plain view: genetic profiling reveals decades old cross contamination of bladder cancer cell line KU7 with HeLa
Purpose: - KU7 is a popular urothelial carcinoma cell line that was isolated from the bladder - of a patient at Keio University in 1980. It has subsequently been widely used in laboratories - around the world. We describe how routine cell line authentication revealed that KU...
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| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
1 Oct 2013
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| In: |
The journal of urology
Year: 2013, Volume: 190, Issue: 4, Pages: 1404-1409 |
| ISSN: | 1527-3792 |
| DOI: | 10.1016/j.juro.2013.03.009 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.juro.2013.03.009 Verlag, lizenzpflichtig, Volltext: https://www.auajournals.org/doi/10.1016/j.juro.2013.03.009 
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| Author Notes: | Wolfgang Jäger, Yutaka Horiguchi, Jay Shah, Tetsutaro Hayashi, Shannon Awrey, Kilian M. Gust, Boris A. Hadaschik, Yoshiyuki Matsui, Shawn Anderson, Robert H. Bell, Susan Ettinger, Alan I. So, Martin E. Gleave, I-Ling Lee, Colin P. Dinney, Masaaki Tachibana, David J. McConkey and Peter C. Black |
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| 245 | 1 | 0 | |a Hiding in plain view |b genetic profiling reveals decades old cross contamination of bladder cancer cell line KU7 with HeLa |c Wolfgang Jäger, Yutaka Horiguchi, Jay Shah, Tetsutaro Hayashi, Shannon Awrey, Kilian M. Gust, Boris A. Hadaschik, Yoshiyuki Matsui, Shawn Anderson, Robert H. Bell, Susan Ettinger, Alan I. So, Martin E. Gleave, I-Ling Lee, Colin P. Dinney, Masaaki Tachibana, David J. McConkey and Peter C. Black |
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| 520 | |a Purpose: - KU7 is a popular urothelial carcinoma cell line that was isolated from the bladder - of a patient at Keio University in 1980. It has subsequently been widely used in laboratories - around the world. We describe how routine cell line authentication revealed that KU7 - was cross contaminated almost 30 years ago with HeLa, a cervical carcinoma cell line. - - Materials and Methods: - Presumed KU7 clones dating from 1984 to 1999 were provided by M.D. Anderson Cancer - Center, Vancouver Prostate Centre, Kyoto University, Tokyo Medical University and - Keio University. HeLa was obtained from ATCC®. Genomic DNA was isolated and short - tandem repeat analysis was performed at the M.D. Anderson Cancer Center Characterized - Cell Line Core Facility, Johns Hopkins University Fragment Analysis Facility and RIKEN - BioResource Center, Ibaraki, Japan. Comparative genomic hybridization was performed - on a platform (Agilent Technologies, Santa Clara, California) at Vancouver Prostate - Centre. - - Results: - The short tandem repeat profile of all KU7 clones was an exact match with that of - HeLa. Comparative genomic hybridization of all samples revealed an abundance of shared - chromosomal aberrations. Slight differences in some genomic areas were explained by - genomic drift in different KU7 clones separated by many years. - - Conclusions: - Our analysis identified that cross contamination of KU7 with HeLa occurred before - 1984 at the source institution. All KU7 clones in the urological literature should - be considered HeLa and experimental results should be viewed in this light. Our results - emphasize the need to authenticate cell lines in oncological research. | ||
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