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Double-strand breaks on YACs during yeast meiosis may reflect meiotic recombination in the human genome

Meiotic recombination in the yeast Saccharomyces cerevisiae is initiated at double-strand breaks (DSBs), which occur preferentially at specific locations1-3. Genetically mapped regions of elevated meiotic recombination (‘hotspots’) coincide with meioticDSB sites, which can be identified on chromosom...

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Hauptverfasser: Klein, Shoshana (VerfasserIn) , Zenvirth, Drora (VerfasserIn) , Sherman, Amir (VerfasserIn) , Ried, Karin (VerfasserIn) , Rappold, Gudrun (VerfasserIn) , Simchen, Giora (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 01 August 1996
In: Nature genetics
Year: 1996, Jahrgang: 13, Heft: 4, Pages: 481-484
ISSN:1546-1718
DOI:10.1038/ng0896-481
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/ng0896-481
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/ng0896-481
Volltext
Verfasserangaben:Shoshana Klein, Drora Zenvirth, Amir Sherman, Karin Ried, Gudrun Rappold & Giora Simchen
Beschreibung
Zusammenfassung:Meiotic recombination in the yeast Saccharomyces cerevisiae is initiated at double-strand breaks (DSBs), which occur preferentially at specific locations1-3. Genetically mapped regions of elevated meiotic recombination (‘hotspots’) coincide with meioticDSB sites, which can be identified on chromosome blots of meiotic DNA (refs 4,5; S.K. et al., manuscript submitted). The morphology of yeast artificial chromosomes (YACs) containing human DNA during the pachytene stage of meiosis resembles that of native yeast chromosomes6. Homologous YAC pairs segregate faithfully and recombine at the high rates characteristic of S. cerevisiae7 (vs. ∼0.4 cM/kb in S. cerevisiae versus ∼10−3 cM/kb in humans). We have examined a variety of YACs carrying human DNA inserts for double-strand breakage during yeast meiosis. Each YAC has a characteristic set of meiotic DSB sites, as do yeast chromosomes (S.K. et al., manuscript submitted). We show that the positions of the DSB sites in the YACs depend on the human-derived DNA in the clones. The degree of double-strand breakage in yeast meiosis of the YACs in our study appears to reflect the degree of meiotic recombination in humans.
Beschreibung:Gesehen am 30.03.2021
Beschreibung:Online Resource
ISSN:1546-1718
DOI:10.1038/ng0896-481

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