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Characterization of a YAC contig spanning the pseudoautosomal region

Due to its unique biology of partial sex linkage and high recombination rates, the pseudoautosomal region (PAR1) on both X and Y chromosomes has attracted considerable interest. In addition, an extremely high level of YAC instability has been observed in this region. We have derived 82 YAC clones fr...

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Main Authors: Ried, Karin (Author) , Mertz, Annelyse (Author) , Nagaraja, Ramaiah (Author) , Trusgnich, Michelle (Author) , Riley, John H. (Author) , Anand, Rakesh (Author) , Lehrach, Hans (Author) , Page, David (Author) , Ellison, Jay W. (Author) , Rappold, Gudrun (Author)
Format: Article (Journal)
Language:English
Published: 1995
In: Genomics
Year: 1995, Volume: 29, Issue: 3, Pages: 787-792
ISSN:1089-8646
DOI:10.1006/geno.1995.9933
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1006/geno.1995.9933
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0888754385799338
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Author Notes:Karin Ried, Annelyse Mertz, Ramaiah Nagaraja, Michelle Trusgnich, John H. Riley, Rakesh Anand, Hans Lehrach, David Page, Jay W. Ellison, and Gudrun Rappold
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Summary:Due to its unique biology of partial sex linkage and high recombination rates, the pseudoautosomal region (PAR1) on both X and Y chromosomes has attracted considerable interest. In addition, an extremely high level of YAC instability has been observed in this region. We have derived 82 YAC clones from six different YAC libraries mapping to this 2.6-Mb region. Of these a subset of 22 YACs was analyzed in detail. YAC contigs were assembled using 67 pseudoautosomal probes, of which 64 were unambiguously ordered. All markers are well distributed over the entire region, including the middle part of the region, which has previously been found difficult to contig. Two gaps of less than 50 kb within the genomic locus of CSF2RA and around XE7 remain, which could not be covered with YACs, cosmids, or phages. This YAC contig anchored on the physical map of PAR1 represents one of the best characterized large regions of the human genome with a map completion greater than 90% at 100-kb resolution and has permitted the accurate localization of all known genes within this region.
Item Description:Online 25 May 2002
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Physical Description:Online Resource
ISSN:1089-8646
DOI:10.1006/geno.1995.9933

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