Cover Image

Protein cost allocation explains metabolic strategies in Escherichia coli

In-depth understanding of microbial growth is crucial for the development of new advances in biotechnology and for combating microbial pathogens. Condition-specific proteome expression is central to microbial physiology and growth. A multitude of processes are dependent on the protein expression, th...

Full description

Saved in:
Bibliographic Details
Main Authors: Grigaitis, Pranas (Author) , Olivier, Brett G. (Author) , Fiedler, Tomas (Author) , Teusink, Bas (Author) , Kummer, Ursula (Author) , Veith, Nadine (Author)
Format: Article (Journal)
Language:English
Published: 2021
In: Journal of biotechnology
Year: 2020, Volume: 327, Pages: 54-63
ISSN:1873-4863
DOI:10.1016/j.jbiotec.2020.11.003
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jbiotec.2020.11.003
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0168165620303047
Get full text
Author Notes:Pranas Grigaitis, Brett G. Olivier, Tomas Fiedler, Bas Teusink, Ursula Kummer, Nadine Veith

MARC

LEADER 00000caa a2200000 c 4500
001 1752969723
003 DE-627
005 20230427070845.0
007 cr uuu---uuuuu
008 210331r20212020xx |||||o 00| ||eng c
024 7 |a 10.1016/j.jbiotec.2020.11.003  |2 doi 
035 |a (DE-627)1752969723 
035 |a (DE-599)KXP1752969723 
035 |a (OCoLC)1341401952 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Grigaitis, Pranas  |e VerfasserIn  |0 (DE-588)1188626469  |0 (DE-627)1667573500  |4 aut 
245 1 0 |a Protein cost allocation explains metabolic strategies in Escherichia coli  |c Pranas Grigaitis, Brett G. Olivier, Tomas Fiedler, Bas Teusink, Ursula Kummer, Nadine Veith 
264 1 |c 2021 
300 |a 10 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Available online 10 December 2020 
500 |a Gesehen am 31.03.2021 
520 |a In-depth understanding of microbial growth is crucial for the development of new advances in biotechnology and for combating microbial pathogens. Condition-specific proteome expression is central to microbial physiology and growth. A multitude of processes are dependent on the protein expression, thus, whole-cell analysis of microbial metabolism using genome-scale metabolic models is an attractive toolset to investigate the behaviour of microorganisms and their communities. However, genome-scale models that incorporate macromolecular expression are still inhibitory complex: the conceptual and computational complexity of these models severely limits their potential applications. In the need for alternatives, here we revisit some of the previous attempts to create genome-scale models of metabolism and macromolecular expression to develop a novel framework for integrating protein abundance and turnover costs to conventional genome-scale models. We show that such a model of Escherichia coli successfully reproduces experimentally determined adaptations of metabolism in a growth condition-dependent manner. Moreover, the model can be used as means of investigating underutilization of the protein machinery among different growth settings. Notably, we obtained strongly improved predictions of flux distributions, considering the costs of protein translation explicitly. This finding in turn suggests protein translation being the main regulation hub for cellular growth. 
534 |c 2020 
650 4 |a Genome-scale models 
650 4 |a Microbial metabolism 
650 4 |a Quantitative proteomics 
650 4 |a Resource allocation 
700 1 |a Olivier, Brett G.  |e VerfasserIn  |0 (DE-588)1189099543  |0 (DE-627)1667835815  |4 aut 
700 1 |a Fiedler, Tomas  |e VerfasserIn  |4 aut 
700 1 |a Teusink, Bas  |e VerfasserIn  |4 aut 
700 1 |a Kummer, Ursula  |d 1967-  |e VerfasserIn  |0 (DE-588)115411682  |0 (DE-627)691297975  |0 (DE-576)176480897  |4 aut 
700 1 |a Veith, Nadine  |e VerfasserIn  |0 (DE-588)1080528016  |0 (DE-627)84476938X  |0 (DE-576)453593062  |4 aut 
773 0 8 |i Enthalten in  |t Journal of biotechnology  |d Amsterdam [u.a.] : Elsevier Science, 1984  |g 327(2021), Seite 54-63  |h Online-Ressource  |w (DE-627)320570851  |w (DE-600)2016476-2  |w (DE-576)090956125  |x 1873-4863  |7 nnas  |a Protein cost allocation explains metabolic strategies in Escherichia coli 
773 1 8 |g volume:327  |g year:2021  |g pages:54-63  |g extent:10  |a Protein cost allocation explains metabolic strategies in Escherichia coli 
856 4 0 |u https://doi.org/10.1016/j.jbiotec.2020.11.003  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.sciencedirect.com/science/article/pii/S0168165620303047  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20210331 
993 |a Article 
994 |a 2021 
998 |g 1080528016  |a Veith, Nadine  |m 1080528016:Veith, Nadine  |d 700000  |d 721000  |e 700000PV1080528016  |e 721000PV1080528016  |k 0/700000/  |k 1/700000/721000/  |p 6  |y j 
998 |g 115411682  |a Kummer, Ursula  |m 115411682:Kummer, Ursula  |d 700000  |d 721000  |e 700000PK115411682  |e 721000PK115411682  |k 0/700000/  |k 1/700000/721000/  |p 5 
998 |g 1188626469  |a Grigaitis, Pranas  |m 1188626469:Grigaitis, Pranas  |d 700000  |d 721000  |e 700000PG1188626469  |e 721000PG1188626469  |k 0/700000/  |k 1/700000/721000/  |p 1  |x j 
999 |a KXP-PPN1752969723  |e 3899600568 
BIB |a Y 
SER |a journal 
JSO |a {"origin":[{"dateIssuedDisp":"2021","dateIssuedKey":"2021"}],"note":["Available online 10 December 2020","Gesehen am 31.03.2021"],"recId":"1752969723","relHost":[{"recId":"320570851","pubHistory":["1.1984 - 168.2013; Vol. 169.2014 -"],"note":["Gesehen am 23.09.09"],"origin":[{"publisherPlace":"Amsterdam [u.a.]","dateIssuedKey":"1984","dateIssuedDisp":"1984-","publisher":"Elsevier Science"}],"id":{"issn":["1873-4863"],"zdb":["2016476-2"],"eki":["320570851"]},"language":["eng"],"disp":"Protein cost allocation explains metabolic strategies in Escherichia coliJournal of biotechnology","physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"Journal of biotechnology","title_sort":"Journal of biotechnology"}],"part":{"volume":"327","extent":"10","year":"2021","pages":"54-63","text":"327(2021), Seite 54-63"},"type":{"bibl":"periodical","media":"Online-Ressource"}}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"name":{"displayForm":["Pranas Grigaitis, Brett G. Olivier, Tomas Fiedler, Bas Teusink, Ursula Kummer, Nadine Veith"]},"title":[{"title":"Protein cost allocation explains metabolic strategies in Escherichia coli","title_sort":"Protein cost allocation explains metabolic strategies in Escherichia coli"}],"person":[{"role":"aut","display":"Grigaitis, Pranas","given":"Pranas","family":"Grigaitis"},{"role":"aut","display":"Olivier, Brett G.","given":"Brett G.","family":"Olivier"},{"display":"Fiedler, Tomas","given":"Tomas","role":"aut","family":"Fiedler"},{"family":"Teusink","display":"Teusink, Bas","given":"Bas","role":"aut"},{"role":"aut","display":"Kummer, Ursula","given":"Ursula","family":"Kummer"},{"family":"Veith","given":"Nadine","display":"Veith, Nadine","role":"aut"}],"id":{"eki":["1752969723"],"doi":["10.1016/j.jbiotec.2020.11.003"]},"physDesc":[{"extent":"10 S."}]} 
SRT |a GRIGAITISPPROTEINCOS2021 

Similar Items