Genetic and structural characterization of the human mitochondrial inner membrane translocase
Translocation of nuclear-encoded mitochondrial preproteins is mediated by translocases in the outer and inner membranes. In the yeast Saccharomyces cerevisiae, translocation of preproteins into the matrix requires the membrane proteins Tim23, Tim17 and Tim44, which drive translocation in cooperation...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
1999
|
| In: |
Journal of molecular biology
Year: 1999, Jahrgang: 289, Heft: 1, Pages: 69-82 |
| ISSN: | 1089-8638 |
| DOI: | 10.1006/jmbi.1999.2751 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1006/jmbi.1999.2751 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0022283699927511 
|
| Verfasserangaben: | Matthias F. Bauer, Klaus Gempel, Andreas S. Reichert, Gudrun A. Rappold, Peter Lichtner, Klaus-Dieter Gerbitz, Walter Neupert, Michael Brunner and Sabine Hofmann |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1753164788 | ||
| 003 | DE-627 | ||
| 005 | 20220819154340.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210406s1999 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1006/jmbi.1999.2751 |2 doi | |
| 035 | |a (DE-627)1753164788 | ||
| 035 | |a (DE-599)KXP1753164788 | ||
| 035 | |a (OCoLC)1341403335 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Bauer, Matthias F. |e VerfasserIn |0 (DE-588)1221299956 |0 (DE-627)1738559734 |4 aut | |
| 245 | 1 | 0 | |a Genetic and structural characterization of the human mitochondrial inner membrane translocase |c Matthias F. Bauer, Klaus Gempel, Andreas S. Reichert, Gudrun A. Rappold, Peter Lichtner, Klaus-Dieter Gerbitz, Walter Neupert, Michael Brunner and Sabine Hofmann |
| 264 | 1 | |c 1999 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Online 25 May 2002 | ||
| 500 | |a Elektronische Reproduktion der Druck-Ausgabe | ||
| 500 | |a Gesehen am 06.04.2021 | ||
| 520 | |a Translocation of nuclear-encoded mitochondrial preproteins is mediated by translocases in the outer and inner membranes. In the yeast Saccharomyces cerevisiae, translocation of preproteins into the matrix requires the membrane proteins Tim23, Tim17 and Tim44, which drive translocation in cooperation with mtHsp70 and its co-chaperone Mge1p. We have cloned and functionally analyzed the human homologues of Tim17, Tim23 and Tim44. In contrast to yeast, two TIM17 genes were found to be expressed in humans. TIM44, TIM23 and TIM17a genes were mapped to chromosomes 19p13.2-p13.3, 10q11.21-q11.23 and 1q32. The TIM17b gene mapped to Xp11.23, near the fusion point where an autosomal region was proposed to have been added to the “ancient” part of the X chromosome about 80-130 MY ago. The primary sequences of the two proteins, hTim17a and hTim17b, are essentially identical, significant differences being restricted to their C termini. They are ubiquitously expressed in fetal and adult tissues, and both show expression levels comparable to that of hTim23. Biochemical characterization of the human Tim components revealed that hTim44 is localized in the matrix and, in contrast to yeast, only loosely associated with the inner membrane. hTim23 is organized into two distinct complexes in the inner membrane, one containing hTim17a and one containing hTim17b. Both TIM complexes display a native molecular mass of 110 kDa. We suggest that the structural organization of TIM23·17 preprotein translocases is conserved from low to high eukaryotes. | ||
| 650 | 4 | |a chromosomal mapping | |
| 650 | 4 | |a human TIM complex | |
| 650 | 4 | |a inner membrane | |
| 650 | 4 | |a mitochondria | |
| 650 | 4 | |a preprotein translocation | |
| 700 | 1 | |a Gempel, Klaus |e VerfasserIn |4 aut | |
| 700 | 1 | |a Reichert, Andreas |d 1969- |e VerfasserIn |0 (DE-588)121633608 |0 (DE-627)705590399 |0 (DE-576)292808089 |4 aut | |
| 700 | 1 | |a Rappold, Gudrun |d 1954- |e VerfasserIn |0 (DE-588)102028319X |0 (DE-627)691169594 |0 (DE-576)359985947 |4 aut | |
| 700 | 1 | |a Lichtner, Peter |d 1967- |e VerfasserIn |0 (DE-588)124154654 |0 (DE-627)085672904 |0 (DE-576)294043675 |4 aut | |
| 700 | 1 | |a Gerbitz, Klaus-Dieter |e VerfasserIn |4 aut | |
| 700 | 1 | |a Neupert, Walter |d 1939-2019 |e VerfasserIn |0 (DE-588)104537217X |0 (DE-627)774469617 |0 (DE-576)39897571X |4 aut | |
| 700 | 1 | |a Brunner, Michael |d 1957- |e VerfasserIn |0 (DE-588)1069891460 |0 (DE-627)822939002 |0 (DE-576)429585004 |4 aut | |
| 700 | 1 | |a Hofmann, Sabine |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of molecular biology |d Amsterdam [u.a.] : Elsevier, 1959 |g 289(1999), 1, Seite 69-82 |h Online-Ressource |w (DE-627)222605472 |w (DE-600)1355192-9 |w (DE-576)094059837 |x 1089-8638 |7 nnas |a Genetic and structural characterization of the human mitochondrial inner membrane translocase |
| 773 | 1 | 8 | |g volume:289 |g year:1999 |g number:1 |g pages:69-82 |g extent:14 |a Genetic and structural characterization of the human mitochondrial inner membrane translocase |
| 856 | 4 | 0 | |u https://doi.org/10.1006/jmbi.1999.2751 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0022283699927511 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210406 | ||
| 993 | |a Article | ||
| 994 | |a 1999 | ||
| 998 | |g 102028319X |a Rappold, Gudrun |m 102028319X:Rappold, Gudrun |d 910000 |d 911500 |e 910000PR102028319X |e 911500PR102028319X |k 0/910000/ |k 1/910000/911500/ |p 4 | ||
| 999 | |a KXP-PPN1753164788 |e 3902136405 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Matthias F. Bauer, Klaus Gempel, Andreas S. Reichert, Gudrun A. Rappold, Peter Lichtner, Klaus-Dieter Gerbitz, Walter Neupert, Michael Brunner and Sabine Hofmann"]},"note":["Online 25 May 2002","Elektronische Reproduktion der Druck-Ausgabe","Gesehen am 06.04.2021"],"recId":"1753164788","person":[{"given":"Matthias F.","family":"Bauer","display":"Bauer, Matthias F.","role":"aut"},{"role":"aut","given":"Klaus","family":"Gempel","display":"Gempel, Klaus"},{"role":"aut","display":"Reichert, Andreas","family":"Reichert","given":"Andreas"},{"role":"aut","display":"Rappold, Gudrun","family":"Rappold","given":"Gudrun"},{"role":"aut","display":"Lichtner, Peter","family":"Lichtner","given":"Peter"},{"family":"Gerbitz","display":"Gerbitz, Klaus-Dieter","given":"Klaus-Dieter","role":"aut"},{"given":"Walter","display":"Neupert, Walter","family":"Neupert","role":"aut"},{"role":"aut","display":"Brunner, Michael","family":"Brunner","given":"Michael"},{"role":"aut","display":"Hofmann, Sabine","family":"Hofmann","given":"Sabine"}],"physDesc":[{"extent":"14 S."}],"title":[{"title_sort":"Genetic and structural characterization of the human mitochondrial inner membrane translocase","title":"Genetic and structural characterization of the human mitochondrial inner membrane translocase"}],"relHost":[{"type":{"bibl":"periodical","media":"Online-Ressource"},"id":{"issn":["1089-8638"],"zdb":["1355192-9"],"eki":["222605472"]},"language":["eng"],"origin":[{"publisherPlace":"Amsterdam [u.a.] ; London","dateIssuedDisp":"1959-","publisher":"Elsevier ; Academic Press","dateIssuedKey":"1959"}],"titleAlt":[{"title":"JMB"}],"title":[{"title":"Journal of molecular biology","subtitle":"JMB","title_sort":"Journal of molecular biology"}],"physDesc":[{"extent":"Online-Ressource"}],"part":{"volume":"289","pages":"69-82","text":"289(1999), 1, Seite 69-82","year":"1999","extent":"14","issue":"1"},"disp":"Genetic and structural characterization of the human mitochondrial inner membrane translocaseJournal of molecular biology","note":["Gesehen am 08.03.11"],"pubHistory":["1.1959 -"],"recId":"222605472"}],"origin":[{"dateIssuedKey":"1999","dateIssuedDisp":"1999"}],"id":{"doi":["10.1006/jmbi.1999.2751"],"eki":["1753164788"]},"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"}} | ||
| SRT | |a BAUERMATTHGENETICAND1999 | ||