Mutational landscape of virus- and UV-associated Merkel cell carcinoma cell lines is comparable to tumor tissue
Merkel cell carcinoma (MCC) is a rare, highly aggressive cutaneous malignancy that is either associated with the integration of the Merkel cell polyomavirus or chronic UV exposure. These two types of carcinogenesis are reflected in characteristic mutational features present in MCC tumor lesions. How...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
5 February 2021
|
| In: |
Cancers
Year: 2021, Jahrgang: 13, Heft: 4, Pages: 1-15 |
| ISSN: | 2072-6694 |
| DOI: | 10.3390/cancers13040649 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/cancers13040649 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2072-6694/13/4/649 
|
| Verfasserangaben: | Kai Horny, Patricia Gerhardt, Angela Hebel-Cherouny, Corinna Wülbeck, Jochen Utikal and Jürgen C. Becker |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1753175283 | ||
| 003 | DE-627 | ||
| 005 | 20220819154552.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210406s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/cancers13040649 |2 doi | |
| 035 | |a (DE-627)1753175283 | ||
| 035 | |a (DE-599)KXP1753175283 | ||
| 035 | |a (OCoLC)1341403466 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Horny, Kai |e VerfasserIn |0 (DE-588)1231016671 |0 (DE-627)1753359139 |4 aut | |
| 245 | 1 | 0 | |a Mutational landscape of virus- and UV-associated Merkel cell carcinoma cell lines is comparable to tumor tissue |c Kai Horny, Patricia Gerhardt, Angela Hebel-Cherouny, Corinna Wülbeck, Jochen Utikal and Jürgen C. Becker |
| 264 | 1 | |c 5 February 2021 | |
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.09.2021 | ||
| 520 | |a Merkel cell carcinoma (MCC) is a rare, highly aggressive cutaneous malignancy that is either associated with the integration of the Merkel cell polyomavirus or chronic UV exposure. These two types of carcinogenesis are reflected in characteristic mutational features present in MCC tumor lesions. However, the genomic characteristics of MCC cell lines used as preclinical models are not well established. Thus, we analyzed the exomes of three virus-negative and six virus-positive MCC cell lines, all showing a classical neuroendocrine growth pattern. Virus-negative cell lines are characterized by a high tumor mutational burden (TMB), UV-light-induced DNA damage, functionally relevant coding mutations, e.g., in RB1 and TP53, and large amounts of copy number variations (CNVs). In contrast, virus-positive cell lines have a low TMB with few coding mutations and lack prominent mutational signatures, but harbor characteristic CNVs. One of the virus-negative cell lines has a local MYC amplification associated with high MYC mRNA expression. In conclusion, virus-positive and -negative MCC cell lines with a neuroendocrine growth pattern resemble mutational features observed in MCC tissue samples, which strengthens their utility for functional studies. | ||
| 650 | 4 | |a cell line | |
| 650 | 4 | |a copy number variation | |
| 650 | 4 | |a merkel cell carcinoma | |
| 650 | 4 | |a merkel cell polyoma virus | |
| 650 | 4 | |a MYC | |
| 650 | 4 | |a RB1 | |
| 650 | 4 | |a significantly mutated genes | |
| 650 | 4 | |a TP53 | |
| 650 | 4 | |a UV | |
| 650 | 4 | |a whole-exome | |
| 700 | 1 | |a Gerhardt, Patricia |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hebel-Cherouny, Angela |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wülbeck, Corinna |d 1966- |e VerfasserIn |0 (DE-588)173037119 |0 (DE-627)697961753 |0 (DE-576)133889866 |4 aut | |
| 700 | 1 | |a Utikal, Jochen |d 1974- |e VerfasserIn |0 (DE-588)1026463750 |0 (DE-627)726765015 |0 (DE-576)371816580 |4 aut | |
| 700 | 1 | |a Becker, Jürgen C. |d 1964- |e VerfasserIn |0 (DE-588)129853437 |0 (DE-627)481957863 |0 (DE-576)216700434 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cancers |d Basel : MDPI, 2009 |g 13(2021), 4, Artikel-ID 649, Seite 1-15 |h Online-Ressource |w (DE-627)614095670 |w (DE-600)2527080-1 |w (DE-576)313958548 |x 2072-6694 |7 nnas |a Mutational landscape of virus- and UV-associated Merkel cell carcinoma cell lines is comparable to tumor tissue |
| 773 | 1 | 8 | |g volume:13 |g year:2021 |g number:4 |g elocationid:649 |g pages:1-15 |g extent:15 |a Mutational landscape of virus- and UV-associated Merkel cell carcinoma cell lines is comparable to tumor tissue |
| 856 | 4 | 0 | |u https://doi.org/10.3390/cancers13040649 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/2072-6694/13/4/649 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210406 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1026463750 |a Utikal, Jochen |m 1026463750:Utikal, Jochen |d 60000 |e 60000PU1026463750 |k 0/60000/ |p 5 | ||
| 998 | |g 1231016671 |a Horny, Kai |m 1231016671:Horny, Kai |d 50000 |e 50000PH1231016671 |k 0/50000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1753175283 |e 3902159227 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Kai Horny, Patricia Gerhardt, Angela Hebel-Cherouny, Corinna Wülbeck, Jochen Utikal and Jürgen C. Becker"]},"person":[{"display":"Horny, Kai","role":"aut","given":"Kai","roleDisplay":"VerfasserIn","family":"Horny"},{"roleDisplay":"VerfasserIn","family":"Gerhardt","role":"aut","display":"Gerhardt, Patricia","given":"Patricia"},{"display":"Hebel-Cherouny, Angela","role":"aut","given":"Angela","roleDisplay":"VerfasserIn","family":"Hebel-Cherouny"},{"roleDisplay":"VerfasserIn","family":"Wülbeck","display":"Wülbeck, Corinna","role":"aut","given":"Corinna"},{"display":"Utikal, Jochen","role":"aut","given":"Jochen","family":"Utikal","roleDisplay":"VerfasserIn"},{"family":"Becker","roleDisplay":"VerfasserIn","role":"aut","display":"Becker, Jürgen C.","given":"Jürgen C."}],"physDesc":[{"extent":"15 S."}],"relHost":[{"recId":"614095670","pubHistory":["1.2009 -"],"id":{"zdb":["2527080-1"],"eki":["614095670"],"issn":["2072-6694"]},"title":[{"title_sort":"Cancers","title":"Cancers"}],"note":["Gesehen am 27.05.2020"],"disp":"Mutational landscape of virus- and UV-associated Merkel cell carcinoma cell lines is comparable to tumor tissueCancers","language":["eng"],"origin":[{"publisher":"MDPI","dateIssuedKey":"2009","publisherPlace":"Basel","dateIssuedDisp":"2009-"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"part":{"extent":"15","year":"2021","pages":"1-15","text":"13(2021), 4, Artikel-ID 649, Seite 1-15","issue":"4","volume":"13"},"name":{"displayForm":["Molecular Diversity Preservation International (MDPI)"]},"physDesc":[{"extent":"Online-Ressource"}]}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedKey":"2021","dateIssuedDisp":"5 February 2021"}],"language":["eng"],"recId":"1753175283","id":{"doi":["10.3390/cancers13040649"],"eki":["1753175283"]},"title":[{"title":"Mutational landscape of virus- and UV-associated Merkel cell carcinoma cell lines is comparable to tumor tissue","title_sort":"Mutational landscape of virus- and UV-associated Merkel cell carcinoma cell lines is comparable to tumor tissue"}],"note":["Gesehen am 08.09.2021"]} | ||
| SRT | |a HORNYKAIGEMUTATIONAL5202 | ||