Molecular analysis of the CDR3 encoding region of the immunoglobulin heavy chain locus in cerebrospinal fluid cells as a diagnostic tool in lymphomatous meningitis
The diagnosis of leptomeningeal B-cell lymphoma is based on the identification of malignant B cells in the cerebrospinal fluid (CSF). Frequently, cytology does not allow clear distinction between neoplastic lymphoid cells and reactively transformed mononuclear cells. Individual B-cell clones can be...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2000
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| In: |
Annals of neurology
Year: 2000, Volume: 47, Issue: 2, Pages: 211-217 |
| ISSN: | 1531-8249 |
| DOI: | 10.1002/1531-8249(200002)47:2<211::AID-ANA11>3.0.CO;2-9 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/1531-8249(200002)47:2<211::AID-ANA11>3.0.CO;2-9 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1531-8249%28200002%2947%3A2%3C211%3A%3AAID-ANA11%3E3.0.CO%3B2-9 
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| Author Notes: | Brigitte Storch‐Hagenlocher, Jürgen Haas, Maria Elisabeth Vogt‐Schaden, Martin Bentz, Lisa Ann Hoffmann, Annette Biessmann, and Brigitte Wildemann |
MARC
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| 520 | |a The diagnosis of leptomeningeal B-cell lymphoma is based on the identification of malignant B cells in the cerebrospinal fluid (CSF). Frequently, cytology does not allow clear distinction between neoplastic lymphoid cells and reactively transformed mononuclear cells. Individual B-cell clones can be identified on the basis of DNA sequences that encode the highly diverse complementarity-determining region (CDR3) of the immunoglobulin heavy chain locus (IgH). We studied CSF samples from 5 patients with B-cell malignancies and cytological evidence of leptomeningeal involvement, using polymerase chain reaction (PCR)-based high-resolution capillary electrophoresis and automated fluorescence analysis to detect PCR fragments. As controls, we assessed CSF specimens from 7 patients with inflammatory neurological diseases and three samples without pathological findings. In all patients with B-cell malignancies, a single PCR product was generated, indicating that CDR3-specific fragments were derived from monoclonal cell populations. CSF samples from patients with inflammatory diseases yielded multiple CDR3 amplicons, suggesting the presence of a polyclonal B-cell activation. No PCR product could be amplified in normal CSF samples. Automated fluorescence detection of CDR3 fragments is a highly sensitive and rapid method to distinguish neoplastic monoclonal and reactive polyclonal B-cell populations in the CSF. Ann Neurol 2000;47:211-217 | ||
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