Superhelical DNA studied by solution scattering and computer models
We present here recent results on the structure of superhelical DNA and its changes with salt concentration between 0.01 and 1.5 M NaCl. Scattering curves of two different superhelical DNAs were determined by static light scattering. The measured radii of gyration do not change significantly with sa...
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| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
June 1999
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| In: |
Genetica
Year: 1999, Jahrgang: 106, Heft: 1, Pages: 49-55 |
| ISSN: | 1573-6857 |
| DOI: | 10.1023/A:1003720610089 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1023/A:1003720610089
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| Verfasserangaben: | Jörg Langowski, Markus Hammermann, Konstantin Klenin, Roland May & Katalin Tóth |
| Zusammenfassung: | We present here recent results on the structure of superhelical DNA and its changes with salt concentration between 0.01 and 1.5 M NaCl. Scattering curves of two different superhelical DNAs were determined by static light scattering. The measured radii of gyration do not change significantly with salt concentration. Small-angle neutron scattering, together with calculations from a Monte Carlo model, allows to determine the superhelix diameter. Measured and simulated scattering curves agreed almost quantitatively. Experimentally we find that the diameter decreases from 16.0±0.9 nm at 10 mM to 9.0±0.7 nm at 100 mM NaCl. The superhelix diameter from the simulated conformations decreased from 18.0±1.5 nm at 10 mM to 9.4±1.5 nm at 100 mM NaCl. At higher salt concentrations up to 1.5 M NaCl, the diameter stays constant at 9 nm. |
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| Beschreibung: | Gesehen am 23.04.2021 |
| Beschreibung: | Online Resource |
| ISSN: | 1573-6857 |
| DOI: | 10.1023/A:1003720610089 |