Correlative light microscopy for high-content screening
High-throughput microscopy is an effective tool for rapidly collecting data on a large scale. However, high throughput comes at the cost of low spatial resolution. Here we introduce correlative light microscopy by combining fast automated widefield imaging, confocal microscopy and super-resolution m...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
November 1, 2013
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| In: |
BioTechniques
Year: 2013, Volume: 55, Issue: 5, Pages: 243-252 |
| ISSN: | 1940-9818 |
| DOI: | 10.2144/000114099 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.2144/000114099 Verlag, lizenzpflichtig, Volltext: https://www.future-science.com/doi/10.2144/000114099 
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| Author Notes: | Benjamin Flottmann, Manuel Gunkel, Tautvydas Lisauskas, Mike Heilemann, Vytaute Starkuviene, Jürgen Reymann, and Holger Erfle |
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| 520 | |a High-throughput microscopy is an effective tool for rapidly collecting data on a large scale. However, high throughput comes at the cost of low spatial resolution. Here we introduce correlative light microscopy by combining fast automated widefield imaging, confocal microscopy and super-resolution microscopy. We demonstrate the potential of this approach for scalable experiments. The workflow consists of a robust approach for selecting cells of interest on a wide-field screening microscope at low resolution and subsequently re-localizing those cells with micrometer precision for confocal and super-resolution imaging. As a case study, we visualized and quantified cis- and trans-Golgi markers at increasing resolution. | ||
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