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(-)-Baclofen-induced and constitutively active inwardly rectifying potassium conductances in cultured rat midbrain neurons

Biophysical and pharmacological properties, and development of the (-)-baclofen-induced potassium (KBac) conductance and the constitutively active inwardly rectifying potassium (KIR) conductance were characterised using the patch-clamp technique in cultured embryonic rat midbrain neurons. KBac condu...

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Hauptverfasser: O'Callaghan, John F. X. (VerfasserIn) , Jarolimek, Wolfgang (VerfasserIn) , Lewen, Andrea (VerfasserIn) , Misgeld, Ulrich (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: November 1996
In: Pflügers Archiv
Year: 1996, Jahrgang: 433, Heft: 1, Pages: 49-57
ISSN:1432-2013
DOI:10.1007/s004240050247
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s004240050247
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Verfasserangaben:J.F.X. O’Callaghan, W. Jarolimek, A. Lewen, U. Misgeld
Beschreibung
Zusammenfassung:Biophysical and pharmacological properties, and development of the (-)-baclofen-induced potassium (KBac) conductance and the constitutively active inwardly rectifying potassium (KIR) conductance were characterised using the patch-clamp technique in cultured embryonic rat midbrain neurons. KBac conductance was induced by (-)-baclofen acting on γ-aminobutyric acid B (GABAB) receptors, and displayed a high degree of selectivity for potassium ions, an approximate square-root dependence of conductance on extracellular potassium concentration and strongly voltage-dependent activation. Ba2+ blocked the conductance in a voltage-independent manner, whereas Cs+ produced a voltage-dependent block. In the same preparation, the KIR conductance displayed biophysical properties indistinguishable from those of the KBac conductance. Block of KIR currents by Ba2+ was voltage independent (KI, 4 μM), whereas Cs+ produced a voltage-dependent block (KI, 370 μM at -100 mV, equivalent valence, z′, 1.67). The KBac and KIR conductances additionally displayed a strikingly similar pattern of development in culture; the specific conductance (nS/pF) of both conductances increased two- to three-fold between the first and second week in vitro, and remained constant thereafter.
Beschreibung:Gesehen am 26.04.2021
Beschreibung:Online Resource
ISSN:1432-2013
DOI:10.1007/s004240050247

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