Stem cell proteomes: a profile of human mesenchymal stem cells derived from umbilical cord blood
Multipotent mesenchymal stem cells (MSCs) derived from human umbilical cord blood (UCB) represent promising candidates for the development of future strategies in cellular therapy. To create a comprehensive protein expression profile for UCB-MSCs, one UCB unit from a full-term delivery was isolated...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
[2005]
|
| In: |
Electrophoresis
Year: 2005, Volume: 26, Issue: 14, Pages: 2749-2758 |
| ISSN: | 1522-2683 |
| DOI: | 10.1002/elps.200410406 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/elps.200410406 Verlag, lizenzpflichtig, Volltext: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/elps.200410406 
|
| Author Notes: | Robert E. Feldmann, Karen Bieback, Martin H. Maurer, Armin Kalenka, Heinrich F. Bürgers, Benjamin Gross, Christian Hunzinger, Harald Klüter, Wolfgang Kuschinsky, Hermann Eichler |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1756736405 | ||
| 003 | DE-627 | ||
| 005 | 20220819191844.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210430s2005 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/elps.200410406 |2 doi | |
| 035 | |a (DE-627)1756736405 | ||
| 035 | |a (DE-599)KXP1756736405 | ||
| 035 | |a (OCoLC)1341407634 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Feldmann, Robert Enrico |d 1970- |e VerfasserIn |0 (DE-588)129555991 |0 (DE-627)47271578X |0 (DE-576)297720554 |4 aut | |
| 245 | 1 | 0 | |a Stem cell proteomes |b a profile of human mesenchymal stem cells derived from umbilical cord blood |c Robert E. Feldmann, Karen Bieback, Martin H. Maurer, Armin Kalenka, Heinrich F. Bürgers, Benjamin Gross, Christian Hunzinger, Harald Klüter, Wolfgang Kuschinsky, Hermann Eichler |
| 264 | 1 | |c [2005] | |
| 300 | |b Diagramme | ||
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 30.04.2021 | ||
| 520 | |a Multipotent mesenchymal stem cells (MSCs) derived from human umbilical cord blood (UCB) represent promising candidates for the development of future strategies in cellular therapy. To create a comprehensive protein expression profile for UCB-MSCs, one UCB unit from a full-term delivery was isolated from the unborn placenta, transferred into culture, and their whole-cell protein fraction was subjected to two-dimensional electrophoresis (2-DE). Unambiguous protein identification was achieved with peptide mass fingerprinting matrix-assisted laser desorption/ionization - time of flight - mass spectrometry (MALDI-TOF-MS), peptide sequencing (MALDI LIFT-TOF/TOF MS), as well as gel-matching with previously identified databases. In overall five replicate 2-DE runs, a total of 2037 ± 437 protein spots were detected of which 205 were identified representing 145 different proteins and 60 isoforms or post-translational modifications. The identified proteins could be grouped into several functional categories, such as metabolism, folding, cytoskeleton, transcription, signal transduction, protein degradation, detoxification, vesicle/protein transport, cell cycle regulation, apoptosis, and calcium homeostasis. The acquired proteome map of nondifferentiated UCB-MSCs is a useful inventory which facilitates the identification of the normal proteomic pattern as well as its changes due to activated or suppressed pathways of cytosolic signal transduction which occur during proliferation, differentiation, or other experimental conditions. | ||
| 650 | 4 | |a Database | |
| 650 | 4 | |a Human mesenchymal stem cells | |
| 650 | 4 | |a Proteomic profiling | |
| 650 | 4 | |a Umbilical cord blood | |
| 700 | 1 | |a Bieback, Karen |d 1972- |e VerfasserIn |0 (DE-588)124509991 |0 (DE-627)376254653 |0 (DE-576)294206604 |4 aut | |
| 700 | 1 | |a Maurer, Martin H. |d 1971- |e VerfasserIn |0 (DE-588)121878112 |0 (DE-627)081593309 |0 (DE-576)182104672 |4 aut | |
| 700 | 1 | |a Kalenka, Armin |d 1969-2025 |e VerfasserIn |0 (DE-588)120095548 |0 (DE-627)080443192 |0 (DE-576)292038798 |4 aut | |
| 700 | 1 | |a Bürgers, Heinrich |d 1971- |e VerfasserIn |0 (DE-588)140530169 |0 (DE-627)618919325 |0 (DE-576)31870353X |4 aut | |
| 700 | 1 | |a Groß, Benjamin |d 1980- |e VerfasserIn |0 (DE-588)143353268 |0 (DE-627)645128090 |0 (DE-576)336604904 |4 aut | |
| 700 | 1 | |a Hunzinger, Christian |d 1970- |e VerfasserIn |0 (DE-588)123324874 |0 (DE-627)706256409 |0 (DE-576)293656150 |4 aut | |
| 700 | 1 | |a Klüter, Harald |e VerfasserIn |0 (DE-588)1026722470 |0 (DE-627)727105132 |0 (DE-576)372016677 |4 aut | |
| 700 | 1 | |a Kuschinsky, Wolfgang |d 1944- |e VerfasserIn |0 (DE-588)1145453708 |0 (DE-627)1005597847 |0 (DE-576)161874711 |4 aut | |
| 700 | 1 | |a Eichler, Hermann |d 1962- |e VerfasserIn |0 (DE-588)133521303 |0 (DE-627)691535388 |0 (DE-576)299904458 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Electrophoresis |d Weinheim : Wiley-Blackwell, 1980 |g 26(2005), 14, Seite 2749-2758 |h Online-Ressource |w (DE-627)26953878X |w (DE-600)1475486-1 |w (DE-576)093885946 |x 1522-2683 |7 nnas |a Stem cell proteomes a profile of human mesenchymal stem cells derived from umbilical cord blood |
| 773 | 1 | 8 | |g volume:26 |g year:2005 |g number:14 |g pages:2749-2758 |g extent:10 |a Stem cell proteomes a profile of human mesenchymal stem cells derived from umbilical cord blood |
| 856 | 4 | 0 | |u https://doi.org/10.1002/elps.200410406 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/elps.200410406 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210430 | ||
| 993 | |a Article | ||
| 994 | |a 2005 | ||
| 998 | |g 133521303 |a Eichler, Hermann |m 133521303:Eichler, Hermann |d 60000 |d 63500 |e 60000PE133521303 |e 63500PE133521303 |k 0/60000/ |k 1/60000/63500/ |p 10 |y j | ||
| 998 | |g 1145453708 |a Kuschinsky, Wolfgang |m 1145453708:Kuschinsky, Wolfgang |d 50000 |d 53200 |e 50000PK1145453708 |e 53200PK1145453708 |k 0/50000/ |k 1/50000/53200/ |p 9 | ||
| 998 | |g 1026722470 |a Klüter, Harald |m 1026722470:Klüter, Harald |d 60000 |d 63500 |d 60000 |e 60000PK1026722470 |e 63500PK1026722470 |e 60000PK1026722470 |k 0/60000/ |k 1/60000/63500/ |k 0/60000/ |p 8 | ||
| 998 | |g 143353268 |a Groß, Benjamin |m 143353268:Groß, Benjamin |d 60000 |d 61600 |e 60000PG143353268 |e 61600PG143353268 |k 0/60000/ |k 1/60000/61600/ |p 6 | ||
| 998 | |g 140530169 |a Bürgers, Heinrich |m 140530169:Bürgers, Heinrich |d 50000 |d 53200 |e 50000PB140530169 |e 53200PB140530169 |k 0/50000/ |k 1/50000/53200/ |p 5 | ||
| 998 | |g 120095548 |a Kalenka, Armin |m 120095548:Kalenka, Armin |d 60000 |d 61600 |e 60000PK120095548 |e 61600PK120095548 |k 0/60000/ |k 1/60000/61600/ |p 4 | ||
| 998 | |g 121878112 |a Maurer, Martin H. |m 121878112:Maurer, Martin H. |d 50000 |d 53200 |d 50000 |e 50000PM121878112 |e 53200PM121878112 |e 50000PM121878112 |k 0/50000/ |k 1/50000/53200/ |k 0/50000/ |p 3 | ||
| 998 | |g 124509991 |a Bieback, Karen |m 124509991:Bieback, Karen |d 60000 |e 60000PB124509991 |k 0/60000/ |p 2 | ||
| 998 | |g 129555991 |a Feldmann, Robert Enrico |m 129555991:Feldmann, Robert Enrico |d 50000 |e 50000PF129555991 |k 0/50000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1756736405 |e 3920413857 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"subtitle":"a profile of human mesenchymal stem cells derived from umbilical cord blood","title_sort":"Stem cell proteomes","title":"Stem cell proteomes"}],"relHost":[{"language":["eng"],"disp":"Stem cell proteomes a profile of human mesenchymal stem cells derived from umbilical cord bloodElectrophoresis","part":{"year":"2005","issue":"14","text":"26(2005), 14, Seite 2749-2758","extent":"10","volume":"26","pages":"2749-2758"},"origin":[{"publisherPlace":"Weinheim ; Weinheim","dateIssuedKey":"1980","dateIssuedDisp":"1980-","publisher":"Wiley-Blackwell ; Wiley-VCH"}],"title":[{"subtitle":"microfluids & proteomics","title_sort":"Electrophoresis","title":"Electrophoresis"}],"corporate":[{"roleDisplay":"Herausgebendes Organ","role":"isb","display":"Electrophoresis Society"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"26953878X","id":{"issn":["1522-2683"],"zdb":["1475486-1"],"eki":["26953878X"],"doi":["10.1002/(ISSN)1522-2683"]},"note":["Gesehen am 10.03.09"],"pubHistory":["1.1980 -"],"physDesc":[{"extent":"Online-Ressource"}]}],"origin":[{"dateIssuedDisp":"[2005]","dateIssuedKey":"2005"}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"doi":["10.1002/elps.200410406"],"eki":["1756736405"]},"recId":"1756736405","physDesc":[{"noteIll":"Diagramme","extent":"10 S."}],"note":["Gesehen am 30.04.2021"],"person":[{"display":"Feldmann, Robert Enrico","role":"aut","roleDisplay":"VerfasserIn","given":"Robert Enrico","family":"Feldmann"},{"display":"Bieback, Karen","role":"aut","roleDisplay":"VerfasserIn","given":"Karen","family":"Bieback"},{"given":"Martin H.","family":"Maurer","display":"Maurer, Martin H.","role":"aut","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","display":"Kalenka, Armin","role":"aut","family":"Kalenka","given":"Armin"},{"given":"Heinrich","family":"Bürgers","display":"Bürgers, Heinrich","role":"aut","roleDisplay":"VerfasserIn"},{"family":"Groß","given":"Benjamin","roleDisplay":"VerfasserIn","role":"aut","display":"Groß, Benjamin"},{"roleDisplay":"VerfasserIn","role":"aut","display":"Hunzinger, Christian","family":"Hunzinger","given":"Christian"},{"roleDisplay":"VerfasserIn","role":"aut","display":"Klüter, Harald","family":"Klüter","given":"Harald"},{"given":"Wolfgang","family":"Kuschinsky","display":"Kuschinsky, Wolfgang","role":"aut","roleDisplay":"VerfasserIn"},{"family":"Eichler","given":"Hermann","roleDisplay":"VerfasserIn","role":"aut","display":"Eichler, Hermann"}],"name":{"displayForm":["Robert E. Feldmann, Karen Bieback, Martin H. Maurer, Armin Kalenka, Heinrich F. Bürgers, Benjamin Gross, Christian Hunzinger, Harald Klüter, Wolfgang Kuschinsky, Hermann Eichler"]}} | ||
| SRT | |a FELDMANNROSTEMCELLPR2005 | ||