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Activation of group I metabotropic glutamate receptors induces long-term depression at sensory synapses in superficial spinal dorsal horn

Low-frequency stimulation of primary afferent Aδ-fibers can induce long-term depression of synaptic transmission in rat superficial spinal dorsal horn. Here, we have identified another form of long-term depression in superficial spinal dorsal horn neurons that is induced by specific group I but not...

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Hauptverfasser: Chen, Jianguo (VerfasserIn) , Heinke, Bernhard (VerfasserIn) , Sandkühler, Jürgen (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 13 December 2000
In: Neuropharmacology
Year: 2000, Jahrgang: 39, Heft: 12, Pages: 2231-2243
ISSN:1873-7064
DOI:10.1016/S0028-3908(00)00084-8
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0028-3908(00)00084-8
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0028390800000848
Volltext
Verfasserangaben:J. Chen, B. Heinke, J. Sandkühler
Beschreibung
Zusammenfassung:Low-frequency stimulation of primary afferent Aδ-fibers can induce long-term depression of synaptic transmission in rat superficial spinal dorsal horn. Here, we have identified another form of long-term depression in superficial spinal dorsal horn neurons that is induced by specific group I but not group II metabotropic glutamate receptor (mGluR) agonists. Synaptic strength between Aδ-fibers and dorsal horn neurons was examined by intracellular recordings in a spinal cord-dorsal root slice preparation from young rat. In the presence of bicuculline and strychnine, bath application of (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid ((1S,3R)-ACPD) or the specific group I mGluR agonist (S)-3,5-dihydroxyphenylglycine ((S)-3,5-DHPG) but not the specific group II mGluR agonist (2S,2′R,3′R)-2-(2′,3′-dicarboxycyclopropyl)glycine (DCG-IV) for 20 min produced an acute and a long-term depression of synaptic strength. Bath application of the N-methyl-d-aspartate receptor antagonist d-2-amino-5-phosphonovaleric acid did not affect these depressions by (1S,3R)-ACPD. After pre-incubation of slices with pertussis toxin, a G-protein inhibitor, (1S,3R)-ACPD still induced acute and long-term depressions. The phospholipase C inhibitor U73122 stereoselectively blocked the induction of long-term depression without affecting acute synaptic inhibition. This study demonstrates that, in the spinal cord, direct activation of group I mGluRs that are coupled to phospholipase C through pertussis toxin-insensitive G-proteins induces a long-term depression of synaptic strength. This may be relevant to the processing of sensory information in the spinal cord, including nociception.
Beschreibung:Gesehen am 03.05.2021
Beschreibung:Online Resource
ISSN:1873-7064
DOI:10.1016/S0028-3908(00)00084-8

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