Improved proteomics-based drug mechanism-of-action studies using 16-plex isobaric mass tags
Multiplexed quantitative proteomics enabled complex workflows to study the mechanisms by which small molecule drugs interact with the proteome such as thermal proteome profiling (TPP) or multiplexed proteome dynamics profiling (mPDP). TPP measures changes in protein thermal stability in response to...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
February 23, 2021
|
| In: |
Journal of proteome research
Year: 2021, Volume: 20, Issue: 3, Pages: 1792-1801 |
| ISSN: | 1535-3907 |
| DOI: | 10.1021/acs.jproteome.0c00900 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acs.jproteome.0c00900
|
| Author Notes: | Nico Zinn, Thilo Werner, Carola Doce, Toby Mathieson, Christine Boecker, Gavain Sweetman, Christian Fufezan, and Marcus Bantscheff |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1757077367 | ||
| 003 | DE-627 | ||
| 005 | 20230426114237.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210504s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/acs.jproteome.0c00900 |2 doi | |
| 035 | |a (DE-627)1757077367 | ||
| 035 | |a (DE-599)KXP1757077367 | ||
| 035 | |a (OCoLC)1341407727 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Zinn, Nico |e VerfasserIn |0 (DE-588)14303555X |0 (DE-627)704370808 |0 (DE-576)334583640 |4 aut | |
| 245 | 1 | 0 | |a Improved proteomics-based drug mechanism-of-action studies using 16-plex isobaric mass tags |c Nico Zinn, Thilo Werner, Carola Doce, Toby Mathieson, Christine Boecker, Gavain Sweetman, Christian Fufezan, and Marcus Bantscheff |
| 246 | 3 | 0 | |a sixteen |
| 264 | 1 | |c February 23, 2021 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 04.05.2021 | ||
| 520 | |a Multiplexed quantitative proteomics enabled complex workflows to study the mechanisms by which small molecule drugs interact with the proteome such as thermal proteome profiling (TPP) or multiplexed proteome dynamics profiling (mPDP). TPP measures changes in protein thermal stability in response to drug treatment and thus informs on direct targets and downstream regulation events, while the mPDP approach enables the discovery of regulated protein synthesis and degradation events caused by small molecules and other perturbations. The isobaric mass tags available for multiplexed proteomics have thus far limited the efficiency and sensitivity by which such experiments could be performed. Here we evaluate a recent generation of 16-plex isobaric mass tags and demonstrate the sensitive and time efficient identification of Staurosporine targets in HepG2 cell extracts by recording full thermal denaturation/aggregation profiles of vehicle and compound treated samples in a single mass spectrometry experiment. In 2D-TPP experiments, isothermal titration over seven concentrations per temperature enabled comprehensive selectivity profiling of Staurosporine with EC50 values for kinase targets tightly matching to the kinobeads gold standard assay. Finally, we demonstrate time and condition-based multiplexing of dynamic SILAC labeling experiments to delineate proteome-wide effects of the molecular glue Indisulam on synthesis and degradation rates. | ||
| 700 | 1 | |a Werner, Thilo |e VerfasserIn |4 aut | |
| 700 | 1 | |a Doce, Carola |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mathieson, Toby |e VerfasserIn |4 aut | |
| 700 | 1 | |a Böcker, Christine |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sweetman, Gavain |e VerfasserIn |4 aut | |
| 700 | 1 | |a Fufezan, Christian |e VerfasserIn |0 (DE-588)1219004677 |0 (DE-627)1734651814 |4 aut | |
| 700 | 1 | |a Bantscheff, Marcus |d 1972- |e VerfasserIn |0 (DE-588)12355263X |0 (DE-627)082625409 |0 (DE-576)293763410 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of proteome research |d Washington, DC : ACS Publications, 2002 |g 20(2021), 3, Seite 1792-1801 |h Online-Ressource |w (DE-627)340077174 |w (DE-600)2065254-9 |w (DE-576)096704608 |x 1535-3907 |7 nnas |a Improved proteomics-based drug mechanism-of-action studies using 16-plex isobaric mass tags |
| 773 | 1 | 8 | |g volume:20 |g year:2021 |g number:3 |g pages:1792-1801 |g extent:10 |a Improved proteomics-based drug mechanism-of-action studies using 16-plex isobaric mass tags |
| 856 | 4 | 0 | |u https://doi.org/10.1021/acs.jproteome.0c00900 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210504 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1219004677 |a Fufezan, Christian |m 1219004677:Fufezan, Christian |d 140000 |e 140000PF1219004677 |k 0/140000/ |p 7 | ||
| 999 | |a KXP-PPN1757077367 |e 3922796818 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"title":[{"title":"Journal of proteome research","title_sort":"Journal of proteome research"}],"note":["Gesehen am 25.08.2020"],"disp":"Improved proteomics-based drug mechanism-of-action studies using 16-plex isobaric mass tagsJournal of proteome research","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"340077174","language":["eng"],"pubHistory":["2002 -"],"part":{"issue":"3","pages":"1792-1801","year":"2021","extent":"10","text":"20(2021), 3, Seite 1792-1801","volume":"20"},"name":{"displayForm":["American Chemical Society"]},"origin":[{"publisherPlace":"Washington, DC","dateIssuedKey":"2002","publisher":"ACS Publications","dateIssuedDisp":"2002-"}],"id":{"issn":["1535-3907"],"zdb":["2065254-9"],"eki":["340077174"]},"physDesc":[{"extent":"Online-Ressource"}]}],"physDesc":[{"extent":"10 S."}],"name":{"displayForm":["Nico Zinn, Thilo Werner, Carola Doce, Toby Mathieson, Christine Boecker, Gavain Sweetman, Christian Fufezan, and Marcus Bantscheff"]},"id":{"doi":["10.1021/acs.jproteome.0c00900"],"eki":["1757077367"]},"origin":[{"dateIssuedKey":"2021","dateIssuedDisp":"February 23, 2021"}],"recId":"1757077367","language":["eng"],"note":["Gesehen am 04.05.2021"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"person":[{"role":"aut","display":"Zinn, Nico","roleDisplay":"VerfasserIn","given":"Nico","family":"Zinn"},{"given":"Thilo","family":"Werner","role":"aut","display":"Werner, Thilo","roleDisplay":"VerfasserIn"},{"family":"Doce","given":"Carola","display":"Doce, Carola","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Mathieson","given":"Toby","roleDisplay":"VerfasserIn","display":"Mathieson, Toby","role":"aut"},{"given":"Christine","family":"Böcker","role":"aut","roleDisplay":"VerfasserIn","display":"Böcker, Christine"},{"given":"Gavain","family":"Sweetman","role":"aut","roleDisplay":"VerfasserIn","display":"Sweetman, Gavain"},{"roleDisplay":"VerfasserIn","display":"Fufezan, Christian","role":"aut","family":"Fufezan","given":"Christian"},{"role":"aut","display":"Bantscheff, Marcus","roleDisplay":"VerfasserIn","given":"Marcus","family":"Bantscheff"}],"title":[{"title_sort":"Improved proteomics-based drug mechanism-of-action studies using 16-plex isobaric mass tags","title":"Improved proteomics-based drug mechanism-of-action studies using 16-plex isobaric mass tags"}]} | ||
| SRT | |a ZINNNICOWEIMPROVEDPR2320 | ||