The HPV-16 E5 protein Inhibits TRAIL- and FasL-Mediated apoptosis in human keratinocyte raft cultures
By using raft cultures of the polyclonal HaCaT cell lines stably transfected either with E5 (HaCaT/E5) or the empty vector (HaCaT/pMSG) as reference, we investigated the effect of the human papillomavirus type 16 (HPV-16) E5 protein on apoptosis. In comparison to conventional monolayer cultures this...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
March 26, 2004
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| In: |
Intervirology
Year: 2004, Jahrgang: 47, Heft: 1, Pages: 48-56 |
| ISSN: | 1423-0100 |
| DOI: | 10.1159/000076642 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1159/000076642 Verlag, lizenzpflichtig, Volltext: https://www.karger.com/Article/FullText/76642 
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| Verfasserangaben: | Kirsten Kabsch, Nina Mossadegh, Annette Kohl, Gerda Komposch, Johannes Schenkel, Angel Alonso, Pascal Tomakidi |
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| 245 | 1 | 4 | |a The HPV-16 E5 protein Inhibits TRAIL- and FasL-Mediated apoptosis in human keratinocyte raft cultures |c Kirsten Kabsch, Nina Mossadegh, Annette Kohl, Gerda Komposch, Johannes Schenkel, Angel Alonso, Pascal Tomakidi |
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| 520 | |a By using raft cultures of the polyclonal HaCaT cell lines stably transfected either with E5 (HaCaT/E5) or the empty vector (HaCaT/pMSG) as reference, we investigated the effect of the human papillomavirus type 16 (HPV-16) E5 protein on apoptosis. In comparison to conventional monolayer cultures this model system allows analysis of apoptosis under more tissue-like conditions by mimicking the stratified organization of a normal surface epithelium. Apoptosis was triggered either by FasL or TRAIL. Execution of the death program was checked at early and late stages by monitoring procaspase-3 cleavage and DNA fragmentation, respectively. Rafts of E5-expressing keratinocytes were completely protected from apoptosis and showed a background of apoptotic cells as low as the untreated cultures. In contrast, the HaCaT/pMSG cultures revealed a dramatic increase in apoptotic cells upon ligand treatment throughout the epithelial compartment. We conclude that the presence of the HPV-16 E5 protein in our tissue-like model prevents FasL- or TRAIL-mediated apoptosis. | ||
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