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Scission of COPI and COPII vesicles is independent of GTP hydrolysis

Synopsis The question as to whether GTP is hydrolyzed for the scission of a coated bud to release a transport vesicle from a donor membrane is pivotal for elucidating the mechanism of the scission step proper. We have revisited the requirement for GTP hydrolysis in the formation of COP vesicles in s...

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Hauptverfasser: Adolf, Frank (Verfasst von) , Herrmann, Alexia (Verfasst von) , Hellwig, Andrea (Verfasst von) , Beck, Rainer (Verfasst von) , Brügger, Britta (Verfasst von) , Wieland, Felix T. (Verfasst von)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 10 June 2013
In: Traffic
Year: 2013, Jahrgang: 14, Heft: 8, Pages: 922-932
ISSN:1600-0854
DOI:https://doi.org/10.1111/tra.12084
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1111/tra.12084
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/tra.12084
Volltext
Verfasserangaben:Frank Adolf, Alexia Herrmann, Andrea Hellwig, Rainer Beck, Britta Brügger and Felix T. Wieland
Beschreibung
Zusammenfassung:Synopsis The question as to whether GTP is hydrolyzed for the scission of a coated bud to release a transport vesicle from a donor membrane is pivotal for elucidating the mechanism of the scission step proper. We have revisited the requirement for GTP hydrolysis in the formation of COP vesicles in semi-intact cells in combination with recombinant COPI and COPII coat proteins and their respective small GTPases (Arf1 and Sar1). Both types of vesicles were efficiently formed and released without the need of GTP hydrolysis or mechanical manipulation.
Beschreibung:Gesehen am 06.05.2021
Beschreibung:Online Resource
ISSN:1600-0854
DOI:https://doi.org/10.1111/tra.12084

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