Scission of COPI and COPII vesicles is independent of GTP hydrolysis
Synopsis The question as to whether GTP is hydrolyzed for the scission of a coated bud to release a transport vesicle from a donor membrane is pivotal for elucidating the mechanism of the scission step proper. We have revisited the requirement for GTP hydrolysis in the formation of COP vesicles in s...
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| Main Authors: | , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
10 June 2013
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| In: |
Traffic
Year: 2013, Volume: 14, Issue: 8, Pages: 922-932 |
| ISSN: | 1600-0854 |
| DOI: | https://doi.org/10.1111/tra.12084 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1111/tra.12084 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/tra.12084 |
| Author Notes: | Frank Adolf, Alexia Herrmann, Andrea Hellwig, Rainer Beck, Britta Brügger and Felix T. Wieland |
MARC
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| 520 | |a Synopsis The question as to whether GTP is hydrolyzed for the scission of a coated bud to release a transport vesicle from a donor membrane is pivotal for elucidating the mechanism of the scission step proper. We have revisited the requirement for GTP hydrolysis in the formation of COP vesicles in semi-intact cells in combination with recombinant COPI and COPII coat proteins and their respective small GTPases (Arf1 and Sar1). Both types of vesicles were efficiently formed and released without the need of GTP hydrolysis or mechanical manipulation. | ||
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