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The human papillomavirus type 11 upstream regulatory region triggers hair-follicle-specific gene expression in transgenic mice

We have generated transgenic mice carrying the URR of the human papillomavirus type 11 ligated in front of the Escherichia coliβ-galactosidase coding region sequence. Using X-Gal staining to demonstrate β-galactosidase production, we observed a hair-specific transcription of the reporter gene. This...

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Hauptverfasser: Schenkel, Johannes (VerfasserIn) , Gaissert, Henning A. (VerfasserIn) , Protopapa, Evangelia (VerfasserIn) , Weiher, Hans (VerfasserIn) , Gissmann, Lutz (VerfasserIn) , Alonso, Angel (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 1999
In: Journal of dermatological science. Supplement
Year: 1999, Jahrgang: 112, Heft: 6, Pages: 893-898
ISSN:1873-569X
DOI:10.1046/j.1523-1747.1999.00589.x
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1046/j.1523-1747.1999.00589.x
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0022202X15405044
Volltext
Verfasserangaben:J. Schenkel, H. Gaissert, E. E. Protopapa, H. Weiher, L. Gissmann, A. Alonso
Beschreibung
Zusammenfassung:We have generated transgenic mice carrying the URR of the human papillomavirus type 11 ligated in front of the Escherichia coliβ-galactosidase coding region sequence. Using X-Gal staining to demonstrate β-galactosidase production, we observed a hair-specific transcription of the reporter gene. This transcription was limited to the epithelial cells of the hair bulge region. The transgene was developmentally regulated, as no LacZ staining was demonstrated during embryogenesis and specific staining was first observed after birth. Surprisingly, dexamethasone and ultraviolet B, but not phorbol myristate acetate or progesterone treatment of the animals resulted in an increase in number and intensity of hair follicles expressing the reporter gene.
Beschreibung:Online 4 January 2016
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Beschreibung:Online Resource
ISSN:1873-569X
DOI:10.1046/j.1523-1747.1999.00589.x

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