Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics
Granule-associated perforin and granzymes (gzms) are key effector molecules of cytotoxic T lymphocytes (Tc cells) and natural killer cells and play a critical role in the control of intracellular pathogens and cancer. Based on the notion that many gzms, including A, B, C, K, H, and M exhibit cytotox...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2010
|
| In: |
The journal of biological chemistry
Year: 2010, Jahrgang: 285, Heft: 24, Pages: 18918-18927 |
| ISSN: | 1083-351X |
| DOI: | 10.1074/jbc.M109.056028 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1074/jbc.M109.056028
|
| Verfasserangaben: | Praxedis Martin, Julián Pardo, Natalie Schill, Lars Jöckel, Matthias Berg, Christopher J. Froelich, Reinhard Wallich, and Markus M. Simon |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1757904239 | ||
| 003 | DE-627 | ||
| 005 | 20230428185605.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210517s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1074/jbc.M109.056028 |2 doi | |
| 035 | |a (DE-627)1757904239 | ||
| 035 | |a (DE-599)KXP1757904239 | ||
| 035 | |a (OCoLC)1341413094 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Martin, Praxedis Sina |e VerfasserIn |0 (DE-588)139288740 |0 (DE-627)703278452 |0 (DE-576)311041515 |4 aut | |
| 245 | 1 | 0 | |a Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics |c Praxedis Martin, Julián Pardo, Natalie Schill, Lars Jöckel, Matthias Berg, Christopher J. Froelich, Reinhard Wallich, and Markus M. Simon |
| 264 | 1 | |c 2010 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 17.05.2021 | ||
| 520 | |a Granule-associated perforin and granzymes (gzms) are key effector molecules of cytotoxic T lymphocytes (Tc cells) and natural killer cells and play a critical role in the control of intracellular pathogens and cancer. Based on the notion that many gzms, including A, B, C, K, H, and M exhibit cytotoxic activity in vitro, all gzms are believed to serve a similar function in vivo. However, more recent evidence supports the concept that gzms are not unidimensional but, rather, possess non-cytotoxic potential, including stimulation of pro-inflammatory cytokines and anti-viral activities. The present study shows that isolated mouse gzmB cleaves the actin-severing mouse protein, cytoplasmic gelsolin (c-gelsolin) in vitro. However, when delivered to intact target cells by ex vivo immune Tc cells, gzmB mediates c-gelsolin proteolysis via activation of caspases 3/7. The NH(2)-terminal c-gelsolin fragment generated by either gzmB or caspase 3 in vitro constitutively severs actin filaments without destroying the target cells. The observation that gzmB secreted by Tc cells initiates a caspase cascade that disintegrates the actin cytoskeleton in target cells suggests that this intracellular process may contribute to anti-viral host defense. | ||
| 650 | 4 | |a Animals | |
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a Caspase 3 | |
| 650 | 4 | |a CD8-Positive T-Lymphocytes | |
| 650 | 4 | |a Cytoskeleton | |
| 650 | 4 | |a Fibroblasts | |
| 650 | 4 | |a Gelsolin | |
| 650 | 4 | |a Granzymes | |
| 650 | 4 | |a Lymphocytic choriomeningitis virus | |
| 650 | 4 | |a Mice | |
| 650 | 4 | |a Microscopy, Fluorescence | |
| 650 | 4 | |a Models, Biological | |
| 650 | 4 | |a RNA, Messenger | |
| 650 | 4 | |a T-Lymphocytes, Cytotoxic | |
| 650 | 4 | |a Transcription, Genetic | |
| 700 | 1 | |a Pardo, Julián |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schill, Natalie |e VerfasserIn |4 aut | |
| 700 | 1 | |a Jöckel, Lars |e VerfasserIn |4 aut | |
| 700 | 1 | |a Berg, Matthias |e VerfasserIn |4 aut | |
| 700 | 1 | |a Froelich, Christopher J. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wallich, Reinhard |e VerfasserIn |0 (DE-588)1059566710 |0 (DE-627)798640839 |0 (DE-576)163467781 |4 aut | |
| 700 | 1 | |a Simon, Markus M. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The journal of biological chemistry |d [Amsterdam] : Elsevier B.V., 1905 |g 285(2010), 24, Seite 18918-18927 |h Online-Ressource |w (DE-627)269247025 |w (DE-600)1474604-9 |w (DE-576)077883837 |x 1083-351X |7 nnas |a Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics |
| 773 | 1 | 8 | |g volume:285 |g year:2010 |g number:24 |g pages:18918-18927 |g extent:10 |a Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics |
| 856 | 4 | 0 | |u https://doi.org/10.1074/jbc.M109.056028 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210517 | ||
| 993 | |a Article | ||
| 994 | |a 2010 | ||
| 998 | |g 1059566710 |a Wallich, Reinhard |m 1059566710:Wallich, Reinhard |d 910000 |d 911600 |e 910000PW1059566710 |e 911600PW1059566710 |k 0/910000/ |k 1/910000/911600/ |p 7 | ||
| 999 | |a KXP-PPN1757904239 |e 3928990446 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics","title_sort":"Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics"}],"person":[{"given":"Praxedis Sina","family":"Martin","role":"aut","roleDisplay":"VerfasserIn","display":"Martin, Praxedis Sina"},{"role":"aut","display":"Pardo, Julián","roleDisplay":"VerfasserIn","given":"Julián","family":"Pardo"},{"given":"Natalie","family":"Schill","role":"aut","roleDisplay":"VerfasserIn","display":"Schill, Natalie"},{"family":"Jöckel","given":"Lars","display":"Jöckel, Lars","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Berg","given":"Matthias","roleDisplay":"VerfasserIn","display":"Berg, Matthias","role":"aut"},{"role":"aut","display":"Froelich, Christopher J.","roleDisplay":"VerfasserIn","given":"Christopher J.","family":"Froelich"},{"given":"Reinhard","family":"Wallich","role":"aut","roleDisplay":"VerfasserIn","display":"Wallich, Reinhard"},{"given":"Markus M.","family":"Simon","role":"aut","display":"Simon, Markus M.","roleDisplay":"VerfasserIn"}],"recId":"1757904239","language":["eng"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 17.05.2021"],"id":{"eki":["1757904239"],"doi":["10.1074/jbc.M109.056028"]},"origin":[{"dateIssuedDisp":"2010","dateIssuedKey":"2010"}],"name":{"displayForm":["Praxedis Martin, Julián Pardo, Natalie Schill, Lars Jöckel, Matthias Berg, Christopher J. Froelich, Reinhard Wallich, and Markus M. Simon"]},"relHost":[{"name":{"displayForm":["publ. by the American Society for Biochemistry and Molecular Biology"]},"id":{"issn":["1083-351X"],"eki":["269247025"],"zdb":["1474604-9"]},"origin":[{"publisherPlace":"[Amsterdam] ; Baltimore, Md. [u.a.] ; Bethesda, Md.","dateIssuedDisp":"2021-","publisher":"Elsevier B.V. ; American Soc. of Biological Chemists ; ASBMB Publications","dateIssuedKey":"2021"}],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"subtitle":"JBC","title":"The journal of biological chemistry","title_sort":"journal of biological chemistry"}],"recId":"269247025","corporate":[{"role":"isb","roleDisplay":"Herausgebendes Organ","display":"American Society for Biochemistry and Molecular Biology"}],"language":["eng"],"note":["Gesehen am 19.05.2021"],"disp":"Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamicsThe journal of biological chemistry","type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"pages":"18918-18927","issue":"24","year":"2010","extent":"10","volume":"285","text":"285(2010), 24, Seite 18918-18927"},"titleAlt":[{"title":"JBC online"},{"title":"JBC"}],"pubHistory":["1.1905/06 -"]}],"physDesc":[{"extent":"10 S."}]} | ||
| SRT | |a MARTINPRAXGRANZYMEBI2010 | ||