An endogene-resembling transgene delays the onset of silencing and limits siRNA accumulation
In plants, transgenes are generally more sensitive against RNA silencing than endogenes are. In this study, we generated a transgene that structurally mimicks an endogene. It is composed of endogenous promoter, 5′-UTR, introns, 3′-UTR and terminator elements. Our data revealed that, in contrast to a...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
1 February 2013
|
| In: |
FEBS letters
Year: 2013, Jahrgang: 587, Heft: 6, Pages: 706-710 |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/j.febslet.2013.01.045 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.febslet.2013.01.045 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0014579313000902 
|
| Verfasserangaben: | Elena Dadami, Mirko Moser, Michele Zwiebel, Gabi Krczal, Michael Wassenegger, Athanasios Dalakouras |
| Zusammenfassung: | In plants, transgenes are generally more sensitive against RNA silencing than endogenes are. In this study, we generated a transgene that structurally mimicks an endogene. It is composed of endogenous promoter, 5′-UTR, introns, 3′-UTR and terminator elements. Our data revealed that, in contrast to a conventional transgene, an endogene-resembling transgene was more stably expressed and poorly processed into small RNAs. In addition, although both constructs triggered methylation of homologous DNA sequences at similar levels, the endogene-resembling transgene exhibited significantly delayed onset of local and systemic silencing. |
|---|---|
| Beschreibung: | Gesehen am 01.06.2021 |
| Beschreibung: | Online Resource |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/j.febslet.2013.01.045 |