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Effects of low concentrated BMP-7 administered by co-cultivation or plasmid transfection on human osteoarthritic chondrocytes

INTRODUCTION: While BMP-7 has proven to be one of the most potent growth factors in cartilage tissue engineering, protein concentration and route of administration remain a matter of debate. Here we investigated the effects of a low concentration of BMP-7 on human osteoarthritic chondrocytes adminis...

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Main Authors: Gavénis, Karsten (Author) , Schneider, Ulrich (Author) , Wallich, Reinhard (Author) , Mueller-Rath, Ralf (Author) , Schmidt-Rohlfing, Bernhard (Author) , Andereya, Stefan (Author)
Format: Article (Journal)
Language:English
Published: 2010
In: The international journal of artificial organs
Year: 2010, Volume: 33, Issue: 6, Pages: 339-347
ISSN:1724-6040
DOI:10.1177/039139881003300602
Online Access:Verlag, Volltext: https://dx.doi.org/10.1177/039139881003300602
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Author Notes:Karsten Gavenis, Ulrich Schneider, Reinhard Wallich, Ralf Mueller-Rath, Bernhard Schmidt-Rohlfing, Stefan Andereya

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245 1 0 |a Effects of low concentrated BMP-7 administered by co-cultivation or plasmid transfection on human osteoarthritic chondrocytes  |c Karsten Gavenis, Ulrich Schneider, Reinhard Wallich, Ralf Mueller-Rath, Bernhard Schmidt-Rohlfing, Stefan Andereya 
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520 |a INTRODUCTION: While BMP-7 has proven to be one of the most potent growth factors in cartilage tissue engineering, protein concentration and route of administration remain a matter of debate. Here we investigated the effects of a low concentration of BMP-7 on human osteoarthritic chondrocytes administered by protein co-cultivation and plasmid transfection. METHODS: Freshly released (P0) or in vitro propagated chondrocytes (P2) were cultivated in a collagen type-I gel for 3 weeks in vitro or in nude mice. Seeded chondrocytes were treated with 50 ng/mL BMP-7 directly added to the medium or were subject to transient BMP-7 plasmid transfection prior to gel cultivation. Untreated specimens served as a control. After recovery, samples were investigated by histological and immunohistochemical staining and real-time PCR. RESULTS: In vitro, collagen type-II protein production was enhanced, and it was stored mainly pericellularly. collagen type-II and aggrecan gene expression were enhanced in both treatment groups. After nude mouse cultivation, col-II protein production was further enhanced, but specimens of the BMP-7 transfection group revealed a clustering of col-II positive cells. Gene expression was strongly upregulated, chondrocyte number was increased and the differentiated phenotype prevailed. In general, freshly released chondrocytes (P0) proved to be superior to chondrocytes pre-amplified in vitro (P2). CONCLUSIONS: Both BMP-7 co-cultivation and plasmid transfection of human osteoarthritic chondrocytes led to improved cartilage repair tissue. Nevertheless, the col-II distribution following BMP-7 co-cultivation was homogeneous, while samples produced by transient transfection revealed a col-II clustering. 
650 4 |a Animals 
650 4 |a Bone Morphogenetic Protein 7 
650 4 |a Cell Culture Techniques 
650 4 |a Chondrocytes 
650 4 |a Coculture Techniques 
650 4 |a Humans 
650 4 |a Lipids 
650 4 |a Mice 
650 4 |a Mice, Inbred BALB C 
650 4 |a Mice, Nude 
650 4 |a Osteoarthritis 
650 4 |a Plasmids 
650 4 |a Transfection 
700 1 |a Schneider, Ulrich  |e VerfasserIn  |4 aut 
700 1 |a Wallich, Reinhard  |e VerfasserIn  |0 (DE-588)1059566710  |0 (DE-627)798640839  |0 (DE-576)163467781  |4 aut 
700 1 |a Mueller-Rath, Ralf  |e VerfasserIn  |4 aut 
700 1 |a Schmidt-Rohlfing, Bernhard  |e VerfasserIn  |4 aut 
700 1 |a Andereya, Stefan  |e VerfasserIn  |4 aut 
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