Conservation of the HBV RNA element epsilon in nackednaviruses reveals ancient origin of protein-primed reverse transcription
Hepadnaviruses, with the human hepatitis B virus as prototype, are small, enveloped hepatotropic DNA viruses which replicate by reverse transcription of an RNA intermediate. Replication is initiated by a unique protein-priming mechanism whereby a hydroxy amino acid side chain of the terminal protein...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
March 22, 2021
|
| In: |
Proceedings of the National Academy of Sciences of the United States of America
Year: 2021, Jahrgang: 118, Heft: 13, Pages: 1-7 |
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.2022373118 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1073/pnas.2022373118 Verlag, lizenzpflichtig, Volltext: https://www.pnas.org/content/118/13/e2022373118 
|
| Verfasserangaben: | Jürgen Beck, Stefan Seitz, Chris Lauber, and Michael Nassal |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1760013803 | ||
| 003 | DE-627 | ||
| 005 | 20220819223333.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210608s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1073/pnas.2022373118 |2 doi | |
| 035 | |a (DE-627)1760013803 | ||
| 035 | |a (DE-599)KXP1760013803 | ||
| 035 | |a (OCoLC)1341415414 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Beck, Jürgen |d 1970- |e VerfasserIn |0 (DE-588)129082007 |0 (DE-627)388712767 |0 (DE-576)297484419 |4 aut | |
| 245 | 1 | 0 | |a Conservation of the HBV RNA element epsilon in nackednaviruses reveals ancient origin of protein-primed reverse transcription |c Jürgen Beck, Stefan Seitz, Chris Lauber, and Michael Nassal |
| 264 | 1 | |c March 22, 2021 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.06.2021 | ||
| 520 | |a Hepadnaviruses, with the human hepatitis B virus as prototype, are small, enveloped hepatotropic DNA viruses which replicate by reverse transcription of an RNA intermediate. Replication is initiated by a unique protein-priming mechanism whereby a hydroxy amino acid side chain of the terminal protein (TP) domain of the viral polymerase (P) is extended into a short DNA oligonucleotide, which subsequently serves as primer for first-strand synthesis. A key component in the priming of reverse transcription is the viral RNA element epsilon, which contains the replication origin and serves as a template for DNA primer synthesis. Here, we show that recently discovered non-enveloped fish viruses, termed nackednaviruses [C. Lauber et al., Cell Host Microbe 22, 387-399 (2017)], employ a fundamentally similar replication mechanism despite their huge phylogenetic distance and major differences in genome organization and viral lifestyle. In vitro cross-priming studies revealed that few strategic nucleotide substitutions in epsilon enable site-specific protein priming by heterologous P proteins, demonstrating that epsilon is functionally conserved since the two virus families diverged more than 400 Mya. In addition, other cis elements crucial for the hepadnavirus-typical replication of pregenomic RNA into relaxed circular double-stranded DNA were identified at conserved positions in the nackednavirus genomes. Hence, the replication mode of both hepadnaviruses and nackednaviruses was already established in their Paleozoic common ancestor, making it a truly ancient and evolutionary robust principle of genome replication that is more widespread than previously thought. | ||
| 650 | 4 | |a HBV long-term evolution | |
| 650 | 4 | |a HBV replication mechanism | |
| 650 | 4 | |a initiation of reverse transcription | |
| 650 | 4 | |a paleovirology | |
| 650 | 4 | |a protein priming | |
| 700 | 1 | |a Seitz, Stefan |e VerfasserIn |0 (DE-588)1110073313 |0 (DE-627)864784627 |0 (DE-576)475719417 |4 aut | |
| 700 | 1 | |a Lauber, Chris |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nassal, Michael |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a National Academy of Sciences (Washington, DC) |t Proceedings of the National Academy of Sciences of the United States of America |d Washington, DC : National Acad. of Sciences, 1915 |g 118(2021), 13 vom: März, Artikel-ID e2022373118, Seite 1-7 |h Online-Ressource |w (DE-627)254235379 |w (DE-600)1461794-8 |w (DE-576)073260509 |x 1091-6490 |7 nnas |
| 773 | 1 | 8 | |g volume:118 |g year:2021 |g number:13 |g month:03 |g elocationid:e2022373118 |g pages:1-7 |g extent:7 |a Conservation of the HBV RNA element epsilon in nackednaviruses reveals ancient origin of protein-primed reverse transcription |
| 856 | 4 | 0 | |u https://doi.org/10.1073/pnas.2022373118 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.pnas.org/content/118/13/e2022373118 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210608 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1110073313 |a Seitz, Stefan |m 1110073313:Seitz, Stefan |d 910000 |d 911700 |e 910000PS1110073313 |e 911700PS1110073313 |k 0/910000/ |k 1/910000/911700/ |p 2 | ||
| 999 | |a KXP-PPN1760013803 |e 3935962797 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Jürgen Beck, Stefan Seitz, Chris Lauber, and Michael Nassal"]},"id":{"eki":["1760013803"],"doi":["10.1073/pnas.2022373118"]},"origin":[{"dateIssuedDisp":"March 22, 2021","dateIssuedKey":"2021"}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"dateIssuedDisp":"1915-","dateIssuedKey":"1915","publisher":"National Acad. of Sciences","publisherPlace":"Washington, DC"}],"id":{"issn":["1091-6490"],"eki":["254235379"],"zdb":["1461794-8"]},"pubHistory":["1.1915 -"],"titleAlt":[{"title":"PNAS online"},{"title":"PNAS"}],"part":{"extent":"7","text":"118(2021), 13 vom: März, Artikel-ID e2022373118, Seite 1-7","volume":"118","pages":"1-7","issue":"13","year":"2021"},"note":["Gesehen am 09.03.2022"],"disp":"National Academy of Sciences (Washington, DC)Proceedings of the National Academy of Sciences of the United States of America","type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"254235379","language":["eng"],"corporate":[{"role":"aut","roleDisplay":"VerfasserIn","display":"National Academy of Sciences"}],"title":[{"title":"Proceedings of the National Academy of Sciences of the United States of America","title_sort":"Proceedings of the National Academy of Sciences of the United States of America"}]}],"physDesc":[{"extent":"7 S."}],"person":[{"given":"Jürgen","family":"Beck","role":"aut","display":"Beck, Jürgen","roleDisplay":"VerfasserIn"},{"given":"Stefan","family":"Seitz","role":"aut","roleDisplay":"VerfasserIn","display":"Seitz, Stefan"},{"family":"Lauber","given":"Chris","roleDisplay":"VerfasserIn","display":"Lauber, Chris","role":"aut"},{"family":"Nassal","given":"Michael","roleDisplay":"VerfasserIn","display":"Nassal, Michael","role":"aut"}],"title":[{"title_sort":"Conservation of the HBV RNA element epsilon in nackednaviruses reveals ancient origin of protein-primed reverse transcription","title":"Conservation of the HBV RNA element epsilon in nackednaviruses reveals ancient origin of protein-primed reverse transcription"}],"recId":"1760013803","language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 08.06.2021"]} | ||
| SRT | |a BECKJUERGECONSERVATI2220 | ||