Aberrant gata-3 expression in human pancreatic cancer
Gata-3 has been shown to specifically alter its expression patterns in different types of cancers. Recent evidence suggests that an interference of Gata-3 exists in the TGF-beta signaling pathway. To determine the role of Gata-3 in pancreatic cancer, pancreatic cancer samples were analyzed in compar...
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| Hauptverfasser: | , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
February 1, 2006
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| In: |
Journal of histochemistry & cytochemistry
Year: 2006, Jahrgang: 54, Heft: 2, Pages: 161-169 |
| ISSN: | 1551-5044 |
| DOI: | 10.1369/jhc.5A6626.200 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1369/jhc.5A6626.200 Verlag, lizenzpflichtig, Volltext: https://journals.sagepub.com/doi/epub/10.1369/jhc.5A6626.2005 
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| Verfasserangaben: | Antanas Gulbinas, Pascal O. Berberat, Zilvinas Dambrauskas, Thomas Giese, Nathalia Giese, Frank Autschbach, Joerg Kleeff, Stefan Meuer, Markus W. Büchler, and Helmut Friess |
| Zusammenfassung: | Gata-3 has been shown to specifically alter its expression patterns in different types of cancers. Recent evidence suggests that an interference of Gata-3 exists in the TGF-beta signaling pathway. To determine the role of Gata-3 in pancreatic cancer, pancreatic cancer samples were analyzed in comparison to normal pancreatic tissues. Furthermore, four different pancreatic cancer cell lines with different alterations of the TGF-beta pathway were studied. To evaluate if a potential relationship with TGF-beta signaling pathway exists, we correlated mRNA expression levels with the expression of TGF-betas, TGF-beta receptors, and Smad-3. Finally, we analyzed the influence of TGF-beta on Gata-3 expression in vitro. All pancreatic cancer samples demonstrated a marked overexpression of Gata-3 mRNA and protein. Immunohistochemical staining revealed strong and persistent cytoplasmic Gata-3 immunoreactivity in cancer cells. In an electrophoretic mobility shift assay, a disturbed nuclear translocation was confirmed. The expression of Gata-3 showed a significant correlation with the expression of TGF-betas, TGF-beta receptors, and Smad-3. TGF-beta responsive cell lines showed a downregulation of Gata-3 mRNA upon TGF-beta exposure, whereas in TGF-beta-unresponsive cell lines, Gata-3 mRNA expression persisted at high levels. Furthermore, strong specific upregulation of Gata-3 impaired nuclear translocation and its cooperative action with the TGF-beta pathway, suggesting that Gata-3 plays a central role in human pancreatic cancer. |
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| Beschreibung: | Gesehen am 09.06.2021 |
| Beschreibung: | Online Resource |
| ISSN: | 1551-5044 |
| DOI: | 10.1369/jhc.5A6626.200 |