A prominent role for mucosal cystine/cysteine metabolism in intestinal immunoregulation
BACKGROUND & AIMS: T-cell receptor reactivity of intestinal lamina propria T cells (LP-T) critically depends on the capacity of local accessory cells to secrete cysteine. For T cells, cysteine is the limiting precursor for glutathione synthesis, a prerequisite for antigen-dependent proliferation...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2008
|
| In: |
Gastroenterology
Year: 2008, Jahrgang: 134, Heft: 1, Pages: 179-191 |
| ISSN: | 1528-0012 |
| DOI: | 10.1053/j.gastro.2007.11.001 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: http://dx.doi.org/10.1053/j.gastro.2007.11.001 Verlag, lizenzpflichtig, Volltext: https://linkinghub.elsevier.com/retrieve/pii/S0016-5085(07)01991-9 
|
| Verfasserangaben: | Bernd Sido, Felix Lasitschka, Thomas Giese, Nikolaus Gassler, Benjamin Funke, Jutta Schröder-Braunstein, Ulf Brunnemer, Stefan C. Meuer, and Frank Autschbach |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1760375535 | ||
| 003 | DE-627 | ||
| 005 | 20230428014412.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210614s2008 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1053/j.gastro.2007.11.001 |2 doi | |
| 035 | |a (DE-627)1760375535 | ||
| 035 | |a (DE-599)KXP1760375535 | ||
| 035 | |a (OCoLC)1341415469 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Sido, Bernd |e VerfasserIn |0 (DE-588)1020440120 |0 (DE-627)688133274 |0 (DE-576)360426859 |4 aut | |
| 245 | 1 | 2 | |a A prominent role for mucosal cystine/cysteine metabolism in intestinal immunoregulation |c Bernd Sido, Felix Lasitschka, Thomas Giese, Nikolaus Gassler, Benjamin Funke, Jutta Schröder-Braunstein, Ulf Brunnemer, Stefan C. Meuer, and Frank Autschbach |
| 264 | 1 | |c 2008 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 14.06.2021 | ||
| 520 | |a BACKGROUND & AIMS: T-cell receptor reactivity of intestinal lamina propria T cells (LP-T) critically depends on the capacity of local accessory cells to secrete cysteine. For T cells, cysteine is the limiting precursor for glutathione synthesis, a prerequisite for antigen-dependent proliferation. We aimed to determine the role of the redoxactive microenvironment for hyporeactivity of LP-T in normal human gut vs hyperreactivity of LP-T in inflammatory bowel disease. METHODS: Parameters relevant to cysteine production, determined as acid-soluble thiol, by intestinal lamina propria macrophages (LP-MO) vs peripheral blood monocytes were investigated (L-[(35)S]cystine uptake via system x(c)(-), messenger RNA, and protein expression of the cystine transporter subunit xCT). Glutathione levels in LP-T and peripheral blood T cells were analyzed both spectrophotometrically and by immunofluorescent staining in situ and in vitro. RESULTS: LP-MO from normal gut, unlike peripheral blood monocytes, are unable to take up cystine, which is due to a deficient expression of the transporter xCT in situ and in vitro. As a consequence, LP-MO do not secrete cysteine. The glutathione content in LP-T from normal gut is <50% of that in autologous peripheral blood T cells. In contrast, in inflammatory bowel disease, CD14(+)CD68(+) LP-MO express xCT and secrete substantial amounts of cysteine upon stimulation, which results in high glutathione levels and full T-cell receptor reactivity in LP-T. CONCLUSIONS: The antioxidative microenvironment of LP-T in inflammatory bowel disease and the prooxidative microenvironment in normal gut explain the differential T-cell receptor reactivities. | ||
| 650 | 4 | |a Case-Control Studies | |
| 650 | 4 | |a Cell Culture Techniques | |
| 650 | 4 | |a Cysteine | |
| 650 | 4 | |a Cystine | |
| 650 | 4 | |a Glutathione | |
| 650 | 4 | |a Humans | |
| 650 | 4 | |a Immunity, Mucosal | |
| 650 | 4 | |a Inflammatory Bowel Diseases | |
| 650 | 4 | |a Intestinal Mucosa | |
| 650 | 4 | |a RNA, Messenger | |
| 650 | 4 | |a T-Lymphocytes | |
| 700 | 1 | |a Lasitschka, Felix |d 1979- |e VerfasserIn |0 (DE-588)140496343 |0 (DE-627)618903542 |0 (DE-576)318526077 |4 aut | |
| 700 | 1 | |a Giese, Thomas |d 1962- |e VerfasserIn |0 (DE-588)143133543 |0 (DE-627)704399075 |0 (DE-576)333724496 |4 aut | |
| 700 | 1 | |a Gaßler, Nikolaus |e VerfasserIn |0 (DE-588)1076884180 |0 (DE-627)835431665 |0 (DE-576)187344329 |4 aut | |
| 700 | 1 | |a Funke, Benjamin |d 1975- |e VerfasserIn |0 (DE-588)128826738 |0 (DE-627)381574881 |0 (DE-576)187502137 |4 aut | |
| 700 | 1 | |a Schröder-Braunstein, Jutta |e VerfasserIn |0 (DE-588)1100387021 |0 (DE-627)859344096 |0 (DE-576)469677821 |4 aut | |
| 700 | 1 | |a Brunnemer, Ulf |d 1981- |e VerfasserIn |0 (DE-588)1027471366 |0 (DE-627)729173666 |0 (DE-576)373246455 |4 aut | |
| 700 | 1 | |a Meuer, Stefan |d 1951- |e VerfasserIn |0 (DE-588)1020440198 |0 (DE-627)691221286 |0 (DE-576)360422454 |4 aut | |
| 700 | 1 | |a Autschbach, Frank |d 1959- |e VerfasserIn |0 (DE-588)1020440090 |0 (DE-627)691221162 |0 (DE-576)360430201 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Gastroenterology |d New York, NY : Elsevier, 1949 |g 134(2008), 1, Seite 179-191 |h Online-Ressource |w (DE-627)270937293 |w (DE-600)1478699-0 |w (DE-576)078590221 |x 1528-0012 |7 nnas |a A prominent role for mucosal cystine/cysteine metabolism in intestinal immunoregulation |
| 773 | 1 | 8 | |g volume:134 |g year:2008 |g number:1 |g pages:179-191 |g extent:13 |a A prominent role for mucosal cystine/cysteine metabolism in intestinal immunoregulation |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1053/j.gastro.2007.11.001 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://linkinghub.elsevier.com/retrieve/pii/S0016-5085(07)01991-9 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210614 | ||
| 993 | |a Article | ||
| 994 | |a 2008 | ||
| 998 | |g 1020440090 |a Autschbach, Frank |m 1020440090:Autschbach, Frank |d 910000 |d 912000 |e 910000PA1020440090 |e 912000PA1020440090 |k 0/910000/ |k 1/910000/912000/ |p 9 |y j | ||
| 998 | |g 1020440198 |a Meuer, Stefan |m 1020440198:Meuer, Stefan |d 910000 |d 911600 |e 910000PM1020440198 |e 911600PM1020440198 |k 0/910000/ |k 1/910000/911600/ |p 8 | ||
| 998 | |g 1027471366 |a Brunnemer, Ulf |m 1027471366:Brunnemer, Ulf |d 910000 |d 912000 |e 910000PB1027471366 |e 912000PB1027471366 |k 0/910000/ |k 1/910000/912000/ |p 7 | ||
| 998 | |g 1100387021 |a Schröder-Braunstein, Jutta |m 1100387021:Schröder-Braunstein, Jutta |d 910000 |d 911600 |e 910000PS1100387021 |e 911600PS1100387021 |k 0/910000/ |k 1/910000/911600/ |p 6 | ||
| 998 | |g 128826738 |a Funke, Benjamin |m 128826738:Funke, Benjamin |d 910000 |d 910300 |e 910000PF128826738 |e 910300PF128826738 |k 0/910000/ |k 1/910000/910300/ |p 5 | ||
| 998 | |g 1076884180 |a Gaßler, Nikolaus |m 1076884180:Gaßler, Nikolaus |p 4 | ||
| 998 | |g 143133543 |a Giese, Thomas |m 143133543:Giese, Thomas |d 910000 |d 911600 |e 910000PG143133543 |e 911600PG143133543 |k 0/910000/ |k 1/910000/911600/ |p 3 | ||
| 998 | |g 140496343 |a Lasitschka, Felix |m 140496343:Lasitschka, Felix |d 910000 |d 912000 |e 910000PL140496343 |e 912000PL140496343 |k 0/910000/ |k 1/910000/912000/ |p 2 | ||
| 998 | |g 1020440120 |a Sido, Bernd |m 1020440120:Sido, Bernd |d 910000 |d 910200 |e 910000PS1020440120 |e 910200PS1020440120 |k 0/910000/ |k 1/910000/910200/ |p 1 |x j | ||
| 999 | |a KXP-PPN1760375535 |e 3938051256 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"13 S."}],"relHost":[{"part":{"year":"2008","pages":"179-191","issue":"1","volume":"134","text":"134(2008), 1, Seite 179-191","extent":"13"},"pubHistory":["Nachgewiesen 12.1949 -"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"American Gastroenterological Association","role":"isb"}],"language":["eng"],"recId":"270937293","disp":"A prominent role for mucosal cystine/cysteine metabolism in intestinal immunoregulationGastroenterology","note":["Gesehen am 21.07.25"],"type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"title_sort":"Gastroenterology","title":"Gastroenterology","subtitle":"official publication of the American Gastroenterological Association"}],"physDesc":[{"extent":"Online-Ressource"}],"id":{"zdb":["1478699-0"],"eki":["270937293"],"issn":["1528-0012"]},"origin":[{"publisherPlace":"New York, NY ; Baltimore, Md. ; Philadelphia, Pa. [u.a.]","dateIssuedDisp":"1949-","dateIssuedKey":"1949","publisher":"Elsevier ; Williams & Wilkins ; Saunders"}]}],"origin":[{"dateIssuedDisp":"2008","dateIssuedKey":"2008"}],"id":{"doi":["10.1053/j.gastro.2007.11.001"],"eki":["1760375535"]},"name":{"displayForm":["Bernd Sido, Felix Lasitschka, Thomas Giese, Nikolaus Gassler, Benjamin Funke, Jutta Schröder-Braunstein, Ulf Brunnemer, Stefan C. Meuer, and Frank Autschbach"]},"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 14.06.2021"],"recId":"1760375535","language":["eng"],"title":[{"title_sort":"prominent role for mucosal cystine/cysteine metabolism in intestinal immunoregulation","title":"A prominent role for mucosal cystine/cysteine metabolism in intestinal immunoregulation"}],"person":[{"role":"aut","roleDisplay":"VerfasserIn","display":"Sido, Bernd","given":"Bernd","family":"Sido"},{"display":"Lasitschka, Felix","roleDisplay":"VerfasserIn","role":"aut","family":"Lasitschka","given":"Felix"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Giese, Thomas","given":"Thomas","family":"Giese"},{"roleDisplay":"VerfasserIn","display":"Gaßler, Nikolaus","role":"aut","family":"Gaßler","given":"Nikolaus"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Funke, Benjamin","given":"Benjamin","family":"Funke"},{"given":"Jutta","family":"Schröder-Braunstein","role":"aut","roleDisplay":"VerfasserIn","display":"Schröder-Braunstein, Jutta"},{"display":"Brunnemer, Ulf","roleDisplay":"VerfasserIn","role":"aut","family":"Brunnemer","given":"Ulf"},{"family":"Meuer","given":"Stefan","display":"Meuer, Stefan","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Autschbach","given":"Frank","roleDisplay":"VerfasserIn","display":"Autschbach, Frank","role":"aut"}]} | ||
| SRT | |a SIDOBERNDLPROMINENTR2008 | ||