Cytokine/chemokine transcript profiles reflect mucosal inflammation in Crohn's disease
BACKGROUND AND AIMS: Immunoregulatory properties of cytokines may contribute to pathological immune reactions in inflammatory bowel disease. There is an urgent need for a simple and dependable means for quantitating inflammatory activity in mucosal biopsies and assessing relapse risk particularly in...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
07 November 2003
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| In: |
International journal of colorectal disease
Year: 2004, Jahrgang: 19, Heft: 4, Pages: 308-315 |
| ISSN: | 1432-1262 |
| DOI: | 10.1007/s00384-003-0554-4 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s00384-003-0554-4
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| Verfasserangaben: | Andreas Stallmach, Thomas Giese, Carsten Schmidt, Bianca Ludwig, Ina Mueller-Molaian & Stefan C. Meuer |
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| 245 | 1 | 0 | |a Cytokine/chemokine transcript profiles reflect mucosal inflammation in Crohn's disease |c Andreas Stallmach, Thomas Giese, Carsten Schmidt, Bianca Ludwig, Ina Mueller-Molaian & Stefan C. Meuer |
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| 520 | |a BACKGROUND AND AIMS: Immunoregulatory properties of cytokines may contribute to pathological immune reactions in inflammatory bowel disease. There is an urgent need for a simple and dependable means for quantitating inflammatory activity in mucosal biopsies and assessing relapse risk particularly in patients with active Crohn's disease (CD). PATIENTS AND METHODS: Cytokine and chemokine transcripts were quantified using real-time PCR in mucosal biopsy specimens from 70 patients with active inflammatory bowel disease (CD, n=45; ulcerative colitis n=25) and 16 patients with specific colitis (ischemic colitis, infectious colitis). Controls were 12 patients with noninflammatory conditions. CD patients with steroid-induced remission (n=20) were followed for up to 12 months. RESULTS: Compared to not-inflamed mucosa the vast majority of active CD tissue samples expressed significantly elevated transcript levels of IL-1beta, IL-8, IL-23, MRP-14, MIP2alpha, and MMP-1. Moreover, increased cytokine transcript levels were detected in both active ulcerative colitis and specific colitis. Importantly, TNF-alpha, IFN-gamma, CD40L, and IL-23 transcripts increased in active CD only. Transcript levels (MRP-14, IL-8, MMP-1, MIP2alpha) were correlated with clinical disease activity (CDAI) and endoscopic scoring indices. Medical treatment induced stable remission in 14 of 20 patients which was paralleled by a reduction in increased transcript levels. All six patients without normalization of MIP2alpha, MRP-14, TNF-alpha, and IL-1beta transcripts developed an early relapse (n=5) or chronic activity (n=1) during follow-up. CONCLUSION: Elevated proinflammatory cytokine transcripts in active CD may underlie disease reactivation and chronicity. Real-time PCR quantification is a simple and objective method for grading inflammation of intestinal mucosa and may be useful in identifying patients who would benefit from anti-inflammatory remission maintenance. | ||
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| 650 | 4 | |a Adult | |
| 650 | 4 | |a Aged | |
| 650 | 4 | |a Aged, 80 and over | |
| 650 | 4 | |a Azathioprine | |
| 650 | 4 | |a Case-Control Studies | |
| 650 | 4 | |a Chemokines | |
| 650 | 4 | |a Crohn Disease | |
| 650 | 4 | |a Cytokines | |
| 650 | 4 | |a Female | |
| 650 | 4 | |a Gene Expression | |
| 650 | 4 | |a Glucocorticoids | |
| 650 | 4 | |a Humans | |
| 650 | 4 | |a Immunosuppressive Agents | |
| 650 | 4 | |a Intestinal Mucosa | |
| 650 | 4 | |a Male | |
| 650 | 4 | |a Middle Aged | |
| 650 | 4 | |a Polymerase Chain Reaction | |
| 650 | 4 | |a Predictive Value of Tests | |
| 650 | 4 | |a Prednisolone | |
| 650 | 4 | |a RNA, Messenger | |
| 650 | 4 | |a Sensitivity and Specificity | |
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