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Proteomic analyses of fibroblast- and serum-derived exosomes identify QSOX1 as a marker for non-invasive detection of colorectal cancer

The treatment of colorectal cancer (CRC) has improved during the last decades, but methods for crucial early diagnosis are yet to be developed. The influence of the tumour microenvironment on liquid biopsies for early cancer diagnostics are gaining growing interest, especially with emphasis on exoso...

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Hauptverfasser: Ganig, Nicole (VerfasserIn) , Baenke, Franziska (VerfasserIn) , Thepkaysone, May-Linn (VerfasserIn) , Lin, Kuailu (VerfasserIn) , Rao, Venkatesh S. (VerfasserIn) , Wong, Fang Cheng (VerfasserIn) , Polster, Heike (VerfasserIn) , Schneider, Martin (VerfasserIn) , Helm, Dominic (VerfasserIn) , Pecqueux, Mathieu (VerfasserIn) , Seifert, Adrian (VerfasserIn) , Seifert, Lena (VerfasserIn) , Weitz, Jürgen (VerfasserIn) , Rahbari, Nuh Nabi (VerfasserIn) , Kahlert, Christoph (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 17 March 2021
In: Cancers
Year: 2021, Jahrgang: 13, Heft: 6, Pages: 1-20
ISSN:2072-6694
DOI:10.3390/cancers13061351
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/cancers13061351
Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2072-6694/13/6/1351
Volltext
Verfasserangaben:Nicole Ganig, Franziska Baenke, May-Linn Thepkaysone, Kuailu Lin, Venkatesh S. Rao, Fang Cheng Wong, Heike Polster, Martin Schneider, Dominic Helm, Mathieu Pecqueux, Adrian M. Seifert, Lena Seifert, Jürgen Weitz, Nuh N. Rahbari and Christoph Kahlert
Beschreibung
Zusammenfassung:The treatment of colorectal cancer (CRC) has improved during the last decades, but methods for crucial early diagnosis are yet to be developed. The influence of the tumour microenvironment on liquid biopsies for early cancer diagnostics are gaining growing interest, especially with emphasis on exosomes (EXO), a subgroup of extracellular vesicles (EVs). In this study, we established paired cancer-associated (CAFs) and normal fibroblasts (NF) from 13 CRC patients and investigated activation status-related protein abundance in derived EXOs. Immunohistochemical staining of matched patient tissue was performed and an independent test cohort of CRC patient plasma-derived EXOs was assessed by ELISA. A total of 11 differentially abundant EV proteins were identified between NFs and CAFs. In plasma EXOs, the CAF-EXO enriched protein EDIL3 was elevated, while the NF-EXO enriched protein QSOX1 was diminished compared to whole plasma. Both markers were significantly reduced in patient-matched CRC tissue compared to healthy colon tissue. In an independent test cohort, a significantly reduced protein abundance of QSOX1 was observed in plasma EXOs from CRC patients compared to controls and diagnostic ROC curve analysis revealed an AUC of 0.904. In conclusion, EXO-associated QSOX1 is a promising novel marker for early diagnosis and non-invasive risk stratification in CRC.
Beschreibung:Gesehen am 19.06.2021
Beschreibung:Online Resource
ISSN:2072-6694
DOI:10.3390/cancers13061351

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