Proteome variations in pancreatic stellate cells upon stimulation with proinflammatory factors*
Pancreatic stellate cells are key mediators in chronic pancreatitis and play a central role in the development of pancreatic fibrosis, stromal formation, and progression of pancreatic cancer. This study was aimed at investigating molecular changes at the level of the proteome that are associated wit...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2013
|
| In: |
The journal of biological chemistry
Year: 2013, Volume: 288, Issue: 45, Pages: 32517-32527 |
| ISSN: | 1083-351X |
| DOI: | 10.1074/jbc.M113.488387 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1074/jbc.M113.488387 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0021925820485837 
|
| Author Notes: | Aseel J. Marzoq, Nathalia Giese, Jörg D. Hoheisel, and Mohamed Saiel Saeed Alhamdani |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1761236288 | ||
| 003 | DE-627 | ||
| 005 | 20230426153114.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210624s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1074/jbc.M113.488387 |2 doi | |
| 035 | |a (DE-627)1761236288 | ||
| 035 | |a (DE-599)KXP1761236288 | ||
| 035 | |a (OCoLC)1341417395 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Marzoq, Aseel J. |e VerfasserIn |0 (DE-588)1235970337 |0 (DE-627)1761236571 |4 aut | |
| 245 | 1 | 0 | |a Proteome variations in pancreatic stellate cells upon stimulation with proinflammatory factors* |c Aseel J. Marzoq, Nathalia Giese, Jörg D. Hoheisel, and Mohamed Saiel Saeed Alhamdani |
| 264 | 1 | |c 2013 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Available online 4 January 2021 | ||
| 500 | |a Gesehen am 24.06.2021 | ||
| 520 | |a Pancreatic stellate cells are key mediators in chronic pancreatitis and play a central role in the development of pancreatic fibrosis, stromal formation, and progression of pancreatic cancer. This study was aimed at investigating molecular changes at the level of the proteome that are associated with the activation of pancreatic stellate cells by proinflammatory factors, namely TNF-α, FGF2, IL6, and chemokine (C-C motif) ligand 4 (CCL4). They were added individually to cells growing in serum-free medium next to controls in medium supplemented with serum, thus containing a mixture of them all, or in serum-free medium alone. Variations were detected by means of a microarray of 810 antibodies targeting relevant proteins. All tested factors triggered increased proliferation and migration. Further analysis showed that TNF-α is the prime factor responsible for the activation of pancreatic stellate cells. CCL4 is associated with cellular neovascularization, whereas FGF2 and IL6 induction led to better cellular survival and decreased apoptotic activity of the stellate cells. The identified direct effects of individual cytokines on human pancreatic stellate cells provide new insights about their contribution to pancreatic cancer promotion. Background: Stroma formation in pancreatic cancer is controlled via the activation of pancreatic stellate cells. Results: Pancreatic stellate cells demonstrate different proteomic and functional attributes following specific stimuli. Conclusion: The proteomic feature of pancreatic stellate cells shows TNF-α as a main contributor to activation. Significance: The shift of proteomes in activated cells may help in understanding the processes of stromal formation and finding new drug targets. | ||
| 650 | 4 | |a Antibody Microarray | |
| 650 | 4 | |a Microarray | |
| 650 | 4 | |a Pancreatic Cancer | |
| 650 | 4 | |a Pancreatic Stellate Cell | |
| 650 | 4 | |a Proteomics | |
| 650 | 4 | |a Stromal Cell | |
| 650 | 4 | |a Tumor Necrosis Factor (TNF) | |
| 700 | 1 | |a Giese, Nathalia |e VerfasserIn |0 (DE-588)1033180955 |0 (DE-627)740120018 |0 (DE-576)380769123 |4 aut | |
| 700 | 1 | |a Hoheisel, Jörg D. |d 1958- |e VerfasserIn |0 (DE-588)1128674645 |0 (DE-627)883097397 |0 (DE-576)486011305 |4 aut | |
| 700 | 1 | |a Alhamdani, Mohamed Saiel Saeed |e VerfasserIn |0 (DE-588)1025391659 |0 (DE-627)72219837X |0 (DE-576)370294505 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The journal of biological chemistry |d [Amsterdam] : Elsevier B.V., 1905 |g 288(2013), 45, Seite 32517-32527 |h Online-Ressource |w (DE-627)269247025 |w (DE-600)1474604-9 |w (DE-576)077883837 |x 1083-351X |7 nnas |a Proteome variations in pancreatic stellate cells upon stimulation with proinflammatory factors* |
| 773 | 1 | 8 | |g volume:288 |g year:2013 |g number:45 |g pages:32517-32527 |g extent:11 |a Proteome variations in pancreatic stellate cells upon stimulation with proinflammatory factors* |
| 856 | 4 | 0 | |u https://doi.org/10.1074/jbc.M113.488387 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0021925820485837 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210624 | ||
| 993 | |a Article | ||
| 994 | |a 2013 | ||
| 998 | |g 1128674645 |a Hoheisel, Jörg D. |m 1128674645:Hoheisel, Jörg D. |d 50000 |e 50000PH1128674645 |k 0/50000/ |p 3 | ||
| 998 | |g 1033180955 |a Giese, Nathalia |m 1033180955:Giese, Nathalia |d 910000 |d 910200 |e 910000PG1033180955 |e 910200PG1033180955 |k 0/910000/ |k 1/910000/910200/ |p 2 | ||
| 999 | |a KXP-PPN1761236288 |e 3941191616 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Proteome variations in pancreatic stellate cells upon stimulation with proinflammatory factors*","title_sort":"Proteome variations in pancreatic stellate cells upon stimulation with proinflammatory factors*"}],"physDesc":[{"extent":"11 S."}],"relHost":[{"title":[{"title_sort":"journal of biological chemistry","subtitle":"JBC","title":"The journal of biological chemistry"}],"id":{"issn":["1083-351X"],"eki":["269247025"],"zdb":["1474604-9"]},"origin":[{"dateIssuedDisp":"2021-","dateIssuedKey":"2021","publisherPlace":"[Amsterdam] ; Baltimore, Md. [u.a.] ; Bethesda, Md.","publisher":"Elsevier B.V. ; American Soc. of Biological Chemists ; ASBMB Publications"}],"corporate":[{"display":"American Society for Biochemistry and Molecular Biology","role":"isb"}],"disp":"Proteome variations in pancreatic stellate cells upon stimulation with proinflammatory factors*The journal of biological chemistry","note":["Gesehen am 19.05.2021"],"titleAlt":[{"title":"JBC online"},{"title":"JBC"}],"pubHistory":["1.1905/06 -"],"part":{"year":"2013","pages":"32517-32527","issue":"45","extent":"11","text":"288(2013), 45, Seite 32517-32527","volume":"288"},"physDesc":[{"extent":"Online-Ressource"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"269247025","name":{"displayForm":["publ. by the American Society for Biochemistry and Molecular Biology"]},"language":["eng"]}],"name":{"displayForm":["Aseel J. Marzoq, Nathalia Giese, Jörg D. Hoheisel, and Mohamed Saiel Saeed Alhamdani"]},"origin":[{"dateIssuedDisp":"2013","dateIssuedKey":"2013"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"recId":"1761236288","id":{"eki":["1761236288"],"doi":["10.1074/jbc.M113.488387"]},"person":[{"given":"Aseel J.","family":"Marzoq","role":"aut","display":"Marzoq, Aseel J."},{"given":"Nathalia","display":"Giese, Nathalia","family":"Giese","role":"aut"},{"role":"aut","family":"Hoheisel","display":"Hoheisel, Jörg D.","given":"Jörg D."},{"given":"Mohamed Saiel Saeed","display":"Alhamdani, Mohamed Saiel Saeed","role":"aut","family":"Alhamdani"}],"language":["eng"],"note":["Available online 4 January 2021","Gesehen am 24.06.2021"]} | ||
| SRT | |a MARZOQASEEPROTEOMEVA2013 | ||