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Border control: a membrane-linked lnteractome of arabidopsis

<p>Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 10<su...

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Hauptverfasser: Jones, Alexander M. (VerfasserIn) , Xuan, Yuanhu (VerfasserIn) , Xu, Meng (VerfasserIn) , Wang, Rui-Sheng (VerfasserIn) , Ho, Cheng-Hsun (VerfasserIn) , Lalonde, Sylvie (VerfasserIn) , You, Chang Hun (VerfasserIn) , Sardi, Maria I. (VerfasserIn) , Parsa, Saman A. (VerfasserIn) , Smith-Valle, Erika (VerfasserIn) , Su, Tianying (VerfasserIn) , Frazer, Keith A. (VerfasserIn) , Pilot, Guillaume (VerfasserIn) , Pratelli, Réjane (VerfasserIn) , Großmann, Guido (VerfasserIn) , Acharya, Biswa R. (VerfasserIn) , Hu, Heng-Cheng (VerfasserIn) , Engineer, Cawas (VerfasserIn) , Villiers, Florent (VerfasserIn) , Ju, Chuanli (VerfasserIn) , Takeda, Kouji (VerfasserIn) , Su, Zhao (VerfasserIn) , Dong, Qunfeng (VerfasserIn) , Assmann, Sarah M. (VerfasserIn) , Chen, Jin (VerfasserIn) , Kwak, June M. (VerfasserIn) , Schroeder, Julian I. (VerfasserIn) , Albert, Reka (VerfasserIn) , Rhee, Seung Y. (VerfasserIn) , Frommer, Wolf B. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 16 May 2014
In: Science
Year: 2014, Jahrgang: 344, Heft: 6185, Pages: 711-716
ISSN:1095-9203
DOI:10.1126/science.1251358
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1126/science.1251358
Verlag, lizenzpflichtig, Volltext: https://science.sciencemag.org/content/344/6185/711
Volltext
Verfasserangaben:Alexander M. Jones, Yuanhu Xuan, Meng Xu, Rui-Sheng Wang, Cheng-Hsun Ho, Sylvie Lalonde, Chang Hun You, Maria I. Sardi, Saman A. Parsa, Erika Smith-Valle, Tianying Su, Keith A. Frazer, Guillaume Pilot, Réjane Pratelli, Guido Grossmann, Biswa R. Acharya, Heng-Cheng Hu, Cawas Engineer, Florent Villiers, Chuanli Ju, Kouji Takeda, Zhao Su, Qunfeng Dong, Sarah M. Assmann, Jin Chen, June M. Kwak, Julian I. Schroeder, Reka Albert, Seung Y. Rhee, Wolf B. Frommer
Beschreibung
Zusammenfassung:<p>Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 10<sup>6</sup> pairs, we identified 12,102 membrane/signaling protein interactions from <i>Arabidopsis</i>. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, >99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split-green fluorescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms.</p>
Beschreibung:Gesehen am 29.06.2021
Beschreibung:Online Resource
ISSN:1095-9203
DOI:10.1126/science.1251358

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