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Purification of high-complexity peptide microarrays by spatially resolved array transfer to gold-coated membranes

A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an...

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Hauptverfasser: Schirwitz, Christopher (VerfasserIn) , Löffler, Felix (VerfasserIn) , Felgenhauer, Thomas (VerfasserIn) , Stadler, Volker (VerfasserIn) , Nesterov-Müller, Alexander (VerfasserIn) , Dahint, Reiner (VerfasserIn) , Breitling, Frank (VerfasserIn) , Bischoff, F. Ralf (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: January 14, 2013
In: Advanced materials
Year: 2013, Jahrgang: 25, Heft: 11, Pages: 1598-1602
ISSN:1521-4095
DOI:10.1002/adma.201203853
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/adma.201203853
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.201203853
Volltext
Verfasserangaben:Christopher Schirwitz, Felix F. Loeffler, Thomas Felgenhauer, Volker Stadler, Alexander Nesterov-Mueller, Reiner Dahint, Frank Breitling, and F. Ralf Bischoff
Beschreibung
Zusammenfassung:A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm2 is demonstrated.
Beschreibung:Gesehen am 29.06.2021
Beschreibung:Online Resource
ISSN:1521-4095
DOI:10.1002/adma.201203853

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