Purification of high-complexity peptide microarrays by spatially resolved array transfer to gold-coated membranes
A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an...
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| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
January 14, 2013
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| In: |
Advanced materials
Year: 2013, Volume: 25, Issue: 11, Pages: 1598-1602 |
| ISSN: | 1521-4095 |
| DOI: | 10.1002/adma.201203853 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/adma.201203853 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.201203853 
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| Author Notes: | Christopher Schirwitz, Felix F. Loeffler, Thomas Felgenhauer, Volker Stadler, Alexander Nesterov-Mueller, Reiner Dahint, Frank Breitling, and F. Ralf Bischoff |
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| 520 | |a A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm2 is demonstrated. | ||
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